Analyses of asiatic hybrids resistance at introduction in northern forest steppe of west Siberia

The contents of main pigments of photosynthetic apparatus ( chlorophyll a, b and carotinoid) in the leaves of Asiatic hybrids varieties during different stage of vegetation was first determined In conditions of northern forest steppe. It was established that 19 varieties of Asiatic hybrids had maximum level of accumulation of chlorophyll pigments (a and b) during the budding period, 4 – during the flowering period, 2 – during the fructification period; the most important value a/b was proper to 8 varieties in the budding period, 6 – in the fructification period and 11 in the flowering period, the maximum accumulation of carotinoind contained in the budding period that was expressed in the adaptive reaction of plants aimed to increase of photosynthetic apparatus resistance during the most dry season period. Analyses of photosynthetic pigment contents in the leaves of Asiatic hybrids varieties showed that the flowing changes in the contents of pigment complex may be considered as indicators of a mechanism of adaptation of lily varieties to the complex of climatic and natural conditions of northern forest steppe of West Siberia.


Introduction
The main task of botanical gardens activity is a search of new plants for its introduction in the culture.Lilium L. genus from Liliaceae Juss.family counts about 105 species and more then 5 thousand varieties.Asiatic hybrids come in the first group of International garden classification of lilies [1,2].They are known as decorative plant which used to create new varieties of lilies.This culture is under study in many regions of Russia and abroad [3][4][5][6][7][8].
Exchange of varieties of Asiatic hybrids between the botanical gardens allows to wide the range of varieties of collections, and to renew genofond.In such a case it's important that the plants adapt well for the complex of natural and climatic conditions of an introduction region.Much value in study of the plants condition has a studying of photosynthetic apparatus flexibility [9,10]  And such parameter analysis gives a definite image on an adaptation of plants to the growth conditions.Firstly the contents of main pigments of photosynthetic apparatus (chlorophyll a, b and carotinoind) contained in the leaves of Asiatic hybrids varieties in different periods of vegetation in the conditions of northern forest steppe of West Siberia was defined and its indicator value.Studies objective: to define the contents of main pigments of photosynthetic apparatus (chlorophyll a, b and carotinoid) contained in the leaves of Asiatic hybrids varieties during different phases of vegetation, to define the varieties resistant tot the complex of natural and climatic conditions on the northern forest steppe of West Siberia.

Results
Normal chlorophyll a, b and carotinoind ratio is: chlorophyll a -50%, chlorophyll b -30%, and carotinoind -20% [13].As L. pilosiusculum is a local species well adapted to the conditions of northern forest steppe of West Siberia, the figures of the pigments content in its photosynthetic apparatus were compared with the introduced varieties of Asiatic hybrids of out of region origin.It was established that 19 varieties of Asiatic Hybrids had maximum accumulation of chlorophyll pigments (a and b) during the budding period, 4 -during the flowering period, and 2 -during the fructification period.The most contents of chlorophyll contained in the leaves of L. pilosiusculum (а, 1,178 ± 0,3 mg/g, b, 0,489 ± 0,03 mg/g).By compare the figures of chlorophyll contents of local species with the introduced it was determined that the contents of chlorophyll in the leaves of ′Lastochka′, ′Pepper′, ′For you′ is near to L. pilosiusculum (а, 0,932-1,563 mg/g, b, 0,318-0,494 mg/g).The contents of chlorophyll in the leaves of ′Rozovaya dymka′ (а, 0,501 mg/g, b, 0,203 mg/g) is in 2 times lower than the local species one.Intraspecific and intravarietal variability of chlorophyll contents is average (V = 0,1-9 %).Quantity of chlorophyll a increased in 2,5-3 times the contents of chlorophyll b in all the phenophases of development during the lily vegetation period.The varieties coefficient of variation for the years of study amounted to V = 0,01-8,37 %.The highest index (V = 8,37 BIO Web of Conferences 11, 00048 (2018) https://doi.org/10.1051/bioconf/20181100048Prospects of Development and Challenges of Modern Botany %) was proper to the variety 'Rozovaya dymka′.′Aelita′ variety showed a stable contents of chlorophyll a and b V = 0,01 %.One on informative figures characterizing the photosynthetic apparatus work is a correlation of chlorophyll a to chlorophyll b (a/b).Such correlation is caused by the «main» chlorophyll a activity.The higher activity increases more the photosynthetic rate.Normally such a figure correspond to 2,2-3,0 mg/g of green weight [14].Following the studies it was determined that 8 varieties had the highest value a/b in the budding period, 6 -in the fructification period and 11 in the flowering period.By compare the average figures a/b it was established that the varieties ′Lastochka′ had the figures (3,281 mg/g), and ′Polymia′ (3,020 mg/g) which are higher than such the figures proper to the variety L. pilosiusculum (2,319 mg/g og green weight).Quantity a/b of all the varieties under study was within the standard values certifying the normal photosynthetic rate.The highest coefficient of a/b variation in the flowering period was proper to the variety ′Fleita′ V = 4,01 %, the lowest V=0,1 % was proper to the variety ′Aelita'.It's known that carotinoid is an important element of lily's pigment system.It was determined that the most part of the introduced varieties of Asiatic hybrids showed the maximum accumulation of carotinoid in the budding period, expressed in the adaptive reaction of the plants aimed to increase resistance of photosynthetic apparatus in more dry season period.Quantitative content of carotinoid in the leaves of L. pilosiusculum (0,368 mg/g) was significant equal to the varieties ′Lastochka′ (0,305 mg/g) and ′Kozette′ (0,393 mg/g) and higher in 1,7 times than contained in the leaves of ′Vostochnaya skazka′ (0,221mg/g).Correlation а+b/carotinoid is no less important in characteristic of photosynthetic apparatus work.This correlation is stable in the norm and is sensitive to any changes in different environment factors.As L. pilosiusculum as the introduced varieties showed a tendency to reduce the correlation a+b/carotinoid near to fructification period that reveal reducing of light-harvesting function of pigment complex under unfavorable temperature conditions impact.At average we got high values a+b/carotinoid demonstrating the good adaptation mechanism of Asiatic hybrids to the environment.Correlation relationship reflects well the plants state as within the usual conditions as within the stresses.Studying intercorrelation of the photosynthetic pigments content we determined that all the varieties have the similar correlation dendrites and the Pleiades order.Total number of authentic correlation relationship (r≥0,601) is variable from 10 to 20.The high level of the parameters conjugation took place in 2013.The close intercorrelation between the studied signs is discernible: carotinoid, chlorophyll a+b, chlorophyll a/b; chlorophyll a and chlorophyll b (fig.1).On the dendrogram of varieties similarity over the pigment content built using the cluster analyses, the species family division is shown.Asiatic hybrids form a single cluster with a shoot height up to 75 cm, another cluster includes the lilies with a culm length up to 75 cm, and the other cluster includes the lilies with a culm length of 80 cm and more (fig.2).

Discussion
Thus, the content of photosynthetic pigments contained in the leaves of Asiatic hybrids showed that the passing changes in the pigment complex structure may be considered as indicators of the mechanism of adaptation of lilies varieties to the complex of natural and climatic conditions on the northern forest steppe of West Siberia.The most varieties meet norms by every measure.It means that the formedness of photosynthetic apparatus of plants and its work ensured availability of a strong protective pigment system that allowed to the Asiatic hybrids varieties to develop well within the vegetation season.Analyses of contemporary foreign literary sources shows that the photosynthetic activity of the eastern hybrids of lilies increases in shade and reduces in water deficit [15,16], i.e. is in complete accord with our opinion concerning protective function of photosynthetic apparatus.Based on the research findings we selected the varieties of Asiatic hybrids with well developed pigment system: lilies of subdivision I. Asiatic hybrids: 'Aelita', 'Virenea', 'Zvezdochka', 'Kamilla', 'Odetta', 'Poliushko', 'Retro', 'Yulia', 'Alaska', 'Gold Lode', 'Pepper', '4 you'.
its adaptation to the varied conditions of introduction.The parameters of accumulation of photosynthetic pigments (chlorophyll a, b, carotinoind) contained in the leaves of Asiatic hybrids during different phase of vegetation BIO Web of Conferences 11, 00048 (2018) https://doi.org/10.1051/bioconf/20181100048Prospects of Development and Challenges of Modern Botany serve as the plants resistance indicators to the complex of natural and climatic conditions.