The results of pre-sowing conifer seeds treatment by growth stimulators

The article studies the influence of different fertilizing products on vigor and germinating ability of Scots pine and common spruce seeds. The research involved the following products: Humate+7, Potassium humate “Sufler”, Biohumus, Agrostimul VE, Emistim-R, Gibberellin, Etamon. The soaking of seeds in products leads to an increase in vigor, on average, between 31 and 37.0 % for Scots pine and between 5 and 13.0 % for common spruce. The germinating ability of Scots pine and common spruce seeds increases, on average, by 16.0 and 17.0 % correspondingly. Among all the products the least effective was “Etamon”. In its case, the germinating ability of seeds in laboratory conditions was 53.0 % which is 10.0 % lower than in control. The treatment by stimulators significantly influenced the development of a root system. The intensive root growth happens in the cases of “Gibberellin” and “Humate+7” – 4.4 and 5 cm corresponding to the variants of the experiment. The pre-sowing treatment positively increases the length of germs. In this case, the best results were achieved with “Gibberellin” and “Biohumus” products that gave the increase of 1.7 and 1.5 cm respectively.


Introduction
The preparation of forest plant seeds for sowing is aimed at overcoming deep or induced dormancy, stimulating mass seed germination and increasing their field germination [1]. Knowing the reasons for dormancy allows for creating and using different techniques of overcoming it by special pre-sowing treatment.
The most common ways to prepare conifer seeds for sowing used in the Republic of Tatarstan and other regions of Russia are storage in snow layers and, sometimes, further pre-sowing treatment by growth activators (soaking).
The pre-sowing treatment is based on a special mechanism: when microelements in growth activators effect on seeds, the plasm of corcule cells is deeply changed which determines the further growth and development of plants. Microelements speed up the germination by 2-4 days, increase the field germination of seeds, their vigor, and stability to unfavorable conditions of the environment and diseases. They also increase the growth and development of plants. The use of microelements increases the liveability of young plants by 20-40 %, especially in bad weather years [2][3][4].
The use of stimulators for growth and development of plants is one of the most important directions to increase the efficiency of young plant growing. At present, the influence of a big number of chemical compounds on different conifer seeds was studied in our country. The most common stimulators used in practice are cobaltous nitrate, copper sulfate, iron, magnesium, manganese, zinc, boric acid, molybdenum ammonium, gibberellin, heteroauxin, petroleum-based growth substance, etc [5][6][7][8]. The pre-sowing treatment of seeds by Verva and Verva-el products at a concentration of 0.1 liter 0.25 mg/kg by means of soaking for 6 hours has led to prolonged influence on the growth indicators in case of pine plantlets. The height of 1-year plantlets was bigger than control by 40-84 %, while the height of 2-year plantlets was bigger by 29-47 %. The diameter of the trunk of 1-year plantlets was bigger by 10-43 % while in the case of 2-year plantlets it was bigger by 11-40 % [9].
When doing the pre-sowing treatment, it is necessary to take into account that effects may be achieved when there is severe lack of used microelements in soil and they are the constraint for young plant growth [10].
The article contains the information about modern ideas about biostimulators used to adjust the growth of plants, reduce the negative effects of abiotic and biotic stress factors and increase the fertility of agricultural plants [11,12].
However, the majority of modern products are tested and used for growing crops. That's why the pre-sowing treatment of conifer seeds is an important research direction in the forestry sector.

Objects and methods of research
The laboratory tests of conifer seed sprouting were carried out between 2018 and 2019. The following products were used to test the efficiency of soaking seeds of Scots pine and common spruce: Humate+7, Potassium humate "Sufler", Biohumus, Agrostimul VE, Emistim-R, Gibberellin, Etamon.
Agrostimul VE (water emulsion) is a highly efficient biological growth and development stimulator for plants. It contains 50 g of dihydroquercetin per liter. It starts to affect after 15-20 minutes after treatment. As a result of treatment, seeds become more immune to diseases and unfavorable environmental factors. It also increases fertility and resistance to diseases.
Emistim-R is a growth stimulator that increases the field germination rate, immunity to diseases and unfavorable environmental factors.
Gibberellin contains a wide range of plant hormones. The most important component is a gibberellic acid that contributes to the active growth of plants.
Etamon is a growth stimulator for plants, a biological plant hormone product of the new generation for stimulating the formation of roots. It can be used for growing plants on open ground and in a greenhouse or under a cover. It can be used for treating seeds and vegetating plants. Primarily, the product stimulates the growth of roots, providing easy-to-digest forms of nitrogen and potassium for bioplasts.
Each variant of the experiment involved 100 seeds and was repeated four times. All work was carried out according to a state standard (GOST 13056.6-97). Trees and shrub seeds. The methods for germination ability determination. The pine and spruce seeds were soaked in products for 24 hours. The experiment involved seeds that were stored for a long time and which sowing quality was decreased. In the case of long-lasting or improper storing the intensity of growth processes decreases and the action of a stimulator becomes more evident [13].
The aim of this research is to study the influence of new fertilizing products on vigor and germinating ability of Scots pine and common spruce seeds.

Results and discussion
Tree and shrub seeds have high physiological activity during development and maturation. Their tissues contain a huge amount of mobile carbohydrates and nitrogen compounds. The germination of seeds starts with their swelling accompanied by splitting and oxidation of fats. Fats are used for the synthesis of organic acids, sugars and amino acids that takes place with the participation of oxidizing and hydrolyzing enzymes [14]. During the research, the first count of Scots pine sprouts was done on the 9th of April. The effect of seeds soaking in fertilizing products was visible already on the 4th day, which is proved by the singlefactor analysis of variance with LSD05 = 2.2. The biggest number of sprouts was given by Emistim-R with the value of 99 %, while the percentage in control variant was at the level of 32 % (table 1). The rest of the product also showed positive effectson sprouting which accounted between 77 and 87 %. The vigor of seeds was estimated on the 7th day of the experiment (11th of April).
There is a positive influence of fertilizers in all variants (LSD05 = 4.24). The least number of sprouts (63 %) was in the case of control. In other cases, involving the fertilizing products the vigor was 94-100 %. The vigor increased, on average, between 31 and 37 % in comparison with control variant ( fig. 1, fig. 2).
On the 15th day, the control showed a relatively low germinating ability of Scots pine seeds equal to 84 %. In other cases, involving the fertilizing products the germinating ability of Scots pine was 96-100%. The biggest germinating ability of seeds was showing with such products as "Emistim-R" (100 %), "Humate+7" (99 %) and "Agrostimul VE" (99 %), which is higher than control by 16 % on average.
The number of empty seeds was determined on the 15th day according to national standards. The biggest amount of empty (10 %) and moldy (6 %) seeds was found in control variant. The linear parameters including the length of roots and the upper part of germs were measured on the 15th day. (see table 2). The biggest value was in case of seed treatment with "Potassium humate" and "Emistim-R". It was equal to 9 cm which is bigger than control by 5 cm. It is worth noting that germs of seeds soaked in products have a different color. They have dark green color which is especially visible when seeds are soaked in such products as "Biohumus" and "Emistem-R". In general, pine seeds have a lower reaction on stimulators in comparison with spruce seeds. On the other hand, the germination of spruce seeds is slower, and the effects are more visible [11]. The following products were used for spruce seeds sprouting: Humate+7, Potassium humate "Sufler", Biohumus, Gibberellin, Etamon. During the research, the first count of spruce sprouts was done on the 19th of May. The biggest number of sprouts was given by Humate+7 with the value of 16 %, while the percentage of sprouts in the control variant was at the level of 1 % (table 3). The rest of the products showed a lesser effect: the number of sprouted seeds was 3-6 %.
The second count of sprouted seeds was done after 3 days on the 22nd of May. The biggest amount of sprouted seeds was in the case of Potassium humate (74 items) which surpasses the control variant by 16.0 %. Among all the products the least effective was "Etamon" (37.0 %). In this case, there is seed depression, as the quantity of sprouted seeds is lower than control by 21.0 %. The vigor of seeds was estimated on the 10th day of the experiment (25th of May) The major part of applied products has prompted the germination on the initial stage which has led to an increase in the vigor. The biggest number of sprouts was in the cases of "Gibberellin" and "Potassium humate" -74 and 77 % respectively. The least number of sprouts was in the case of "Etamon" -48 % against 61 % in control. The final results of sprouting the seeds of common spruce were obtained on the 30th of May. The biggest number of sprouts was in the cases of "Potassium humate" and "Gibberellin" -74-80 % (LSD05 = 1.68) which is higher than control by 11-17 %. The same trend as in the case of seed vigor accounting was on the 15th day. All variants showed a positive influence of products, except "Etamon". In its case, the germination The number of empty seeds was determined on the 15th day according to national standards. The biggest amount of empty seeds (32 %) was found in the control variant. The seeds with small amounts of mold and rottenness (1-3 %) were also accounted except the variant with "Etamon", where mold accounted for 8.0 % and rottenness for 10 %.
The research also involved the measurement of common spruce sprouts length (table 3). Table 4 shows that pre-sowing treatment positively increases the length of sprouts in comparison with untreated seeds. On the 7th day, the longest spruce sprouts were in the cases of "Gibberellin" and "Biohumus" with 1.7 and 1.5 cm respectively. On the 10th day, there was the same trend with the biggest values obtained in the same variants with 3.0 and 3.5 cm respectively. The same trend preserves in case of other variants using growth stimulators.
The linear parameters including the length of roots and the upper part of germs were measured separately on the 15th day. (see table 5) The biggest values were obtained in the cases of "Potassium humate" and "Humate+7" with 10.5 and 11.5 values respectively, which surpasses the control by 5.5 and 6.5 cm respectively.
The treatment by stimulators significantly influenced the development of a root system. The intensive root growth happens in the cases of "Gibberellin" and "Humate+7" -4.4 and 5 cm corresponding to the variants of the experiment. This is a big advantage when growing trees in forest nurseries because the growth and development of sprouts depend on the development of root systems.

Conclusion
The laboratory experiments on the pre-sowing treatment of conifer seeds showed that: • soaking of seeds in fertilizing products leads to the increase in vigor, on average, between 31 and 37.0 % for Scots pine and between 5 and 13.0 % for common spruce; • germinating ability of Scots pine and common spruce seeds increases, on average, by 16.0 % and 17.0 % correspondingly.
The pre-sowing treatment of seeds with fertilizing products has a high potential. However, to fix the results it is necessary to conduct field experiments in nurseries and greenhouses of the Republic of Tatarstan. Potassium humate Humate+7 Biohumus Gibberellin Etamon