Productivity and quality indicators of potato tubers using bioagents and adaptogenic drugs

Studies to identify the effectiveness of bioagents and adaptogenic preparations on plantings of potatoes of the Vineta variety were carried out on gray forest medium loam granulometric composition of the soil of the Zemlyaki farm in the Nizhnekamsk district of the Republic of Tatarstan. Field experiments were carried out in 2019 by employees of the Department of Plant Growing and Horticulture of Kazan State Agrarian University. The humus content in the arable layer of soil according to Tyurin is 3.35 %, pH is 5.7, easily hydrolyzable nitrogen is 112 mg/kg of soil, mobile phosphorus is 156 mg/kg, exchange potassium is 136 mg/kg of soil, molybdenum is 0.07, copper is 0.52, boron – 0.7, zinc – 4.82 mg/kg. The paper presents the results of studies on the effect of the preparation RECB-95B obtained on the basis of bacteria Bacillus subtilus, RECB-44 – Pseudomonas fluorescens, RECB-50B – Bacillus sp. and RECB-74B — derived from the fungus Trichoderma viride for antagonistic activity against pathogens. Based on the results of our preliminary studies, we selected these preparations for preplant treatment of tubers and vegetative plants. It was found that the most effective option for planting potatoes was the treatment of tubers before planting with the strain RECB-50B (2 l/t) + adaptogen in combination with three times spraying during the growing season RECB-95B, RECB-4B, RECB-50B using them together with adaptogen. Our data show that the mass of tubers does not always correspond to the number of stems and tubers, however, in variants 7 and 8 with a high mass of tubers, a larger number of stems and tubers per bush was noted. Less nitrates 73 mg/kg contained tubers from the variant when used for processing tubers of strain RECB – 50 B (2 l/t) and triple treatment of plants during the growing season with bioagents RECB – 95 B (0.5 l/ha), RECB – 44 B (0.5 l/ha), RECB – 50 B (1.0 l/ha). The use of these drugs in conjunction with the adaptogen led to an increase in the number of nitrates in the tubers. A higher starch content in tubers of 13.02 and 13.01 % was observed when treating the tubers of the drug Maxim (0.2 l/t) and strain RECB – 50 B (2 l/t) and triple treatment of plants during the growing season with bioagents RECB – 95 B (0.5 l/ha ), RECB – 44 B (0.5 l/ha), RECB – 50 B (1.0 l/ha). In the dry matter, the starch content was higher when the tubers were treated with rhizoplan and amounted to 63.38 %.


Introduction
Potato harvest is formed during photosynthesis, meaning in the process of absorbing light and transforming its energy into the chemical potential of energy-rich organic compounds in the form of carbohydrates, fats, proteins. The productivity of potato plants largely depends on photosynthetic activity. This includes many indicators and the dynamic ratio of leaf area, the duration of their life, the intensity of the photosynthesis process itself. The most important factors affecting the growth and development of plants is solar radiation, which is the energy basis of plant life. This factor, unlike others, such as mineral nutrition, water and temperature conditions, carbon dioxide content is the most difficult to control.
Based on the data obtained, several researchers note that the optimal leaf area is 30-40 thousand m2/ha, at which the proportion of absorbed solar energy increases significantly. In the works of other researchers, the best results were obtained with leaf areas of 50 and more than thousand m 2 /ha. However, they note that excessive excess of the leaf area from the optimal level leads to a decrease in crop accumulation per unit area of leaves.
Crop production, including the cultivation of potatoes in modern conditions, is carried out in conditions of global natural and man-made pressure. It is associated with significant climate change, xenobiotic pollution, and unsustainable moisture supply. As a result, there is a decrease in sown areas, a decrease in plant resistance to adverse environmental factors, and the formation of resistant strains of pathogenic microorganisms.
One of the main directions contributing to the improvement of the environmental situation is the use of biological preparations that can stimulate the immune system and increase resistance to diseases. The basis of biofungicides is composed of strains of rhizospheric bacteria Bacillus subtilis, which during their growth can synthesize substances that inhibit the development of phytopathogenic fungi and bacteria that are causative agents of plant diseases [1,2].
It has now been established that biological preparations increase plant resistance to adverse environmental factors and various fungal diseases. Along with this leads to increased crop yields [3][4][5].
In this regard, in the laboratory of the Kazan State Agrarian University, innovative research and production studies of biological preparations are developed and carried out, which will raise the potato industry to a higher level.

The purpose and objectives of research
The aim of the work is the theoretical justification and development of practical methods for pre-planting treatment of potato seeds in combination with leaf dressing of vegetative plants with bioagents and adaptogenic preparations for planting potatoes of the Vineta variety.
To achieve this goal, the following tasks were envisaged: • conduct a comparative assessment of the effect of doses of mineral fertilizers and biological products on the yield and quality of potato tubers.

Research Program and Methodology
The studies were conducted on gray forest medium loam granulometric composition of the farm Zemlyaki of the Nizhnekamsk district of the Republic of Tatarstan. The humus content in the arable layer of soil according to Tyurin is 3.35 %, pH is 5.7, easily hydrolyzable nitrogen is 112 mg/kg of soil, mobile phosphorus is 156 mg/kg, exchange potassium is 136 mg/kg of soil, molybdenum is 0.07, copper is 0.52, boron -0.7, zinc -4.82 mg/kg.
The predecessor of the facility was winter wheat. In the experiment, a cultivar of Vineta potato was cultivated. Mineral fertilizers (nitroammophosk 2 c/ha + ammonium nitrate 1 c/ha) were introduced during planting. An extract from millet seedlings was used as an adaptogen.
For planting, seed tubers of the first reproduction with a mass of 60...65 g were used. Aisle spacing was 75 cm, planting density was 44.33 thousand tubers per ha (30 × 75 cm). Planting depth 10...12 cm, the total area of the plot was 72 m 2 , accounting 60 m 2 . Autumn soil preparation consisted of stubble cultivation with disc cultivators to a depth of 6...8 cm after harvesting the predecessor, and after 10...12 days plowing with reversible plows. In spring, milling vertically with a milling cultivator Zirkon -7/300. Planting was performed with a four-row potato planter Hassia SL 4 BZS.
The studies were carried out with the financial support of the Ministry of Education and Science of the Russian Federation in the framework of the Federal Center for Strategic Research "Research in the priority areas for the development of the scientific and technical complex of Russia for 2014-2020", agreement number 14.620.21.0017, unique project identification number -RFMEF161017X0017.
The studies were carried out in accordance with the methods described in the textbooks of V.F. Moseychenko, [7]. Statistical processing of research results was carried out by analysis of variance [8].

Results
To protect plants among microorganisms, an important place is occupied by Bacillus bacteria, which exhibit antagonistic activity against a significant number of pests and pathogens. They also have a positive effect on crop immunity. Given all this, bacteria of this genus were included in the bioagents we are studying.
The potato plant is characterized by the height of the bush, the ratio of the mass of stems and leaves, the size of the leaf surface. The symbiosis between plants and microorganisms was manifested in the growth indicators of potato plants. An analysis of the data showed that, compared to the application for the treatment of rhizoplan tubers, the use of the bio-agent RECB -50 B D.R. Noosey [10] notes that this factor is substantially determined by storage temperature. Storage at temperatures above 7 °C promotes early exit of tubers from dormancy and enhances apical dominance. When planting such planting material, plants quickly grow and develop, mature earlier, but have fewer stems, and therefore tubers, whose mass is relatively high. When storing planting material at lower temperatures (below 5 °C), it was accompanied by a weakening of apical dominance, delayed germination, growth and development, and an increase in the number of stems and small tubers.
In the literature, some researchers studying the structure of the crop of potato tubers note that the number of tubers depends on the number of stems. Our data show that this phenomenon does not always coincide, however, in variants 7 and 8, with a high mass of tubers, a larger number of stems and tubers per one bush was noted. On these options was the largest aboveground mass and weight of leaves per 1 bush (Table 2). The In the formation of a high economic potato crop, several factors are crucial. These include the speed with which the assimilation apparatus is formed, the size of the active leaf surface, leaf productivity, leaf life, relative growth rate of storage organs.
However, opinions on which of the above factors of the development of a potato plant play a decisive role differ. K.H. Engel [11] notes the dominant role of the leaves, other rate of tuber formation. These differences seem to have no basis. These factors influence not fragmentedly but are in interaction with each other.
Growth duration depended on meteorological conditions of the year. If two irrigation was used in the first half of the growing season, then in the second half, when a significant amount of rainfall occurred, the leaf functioning and tuber formation duration was extended, which positively affected the potato yield (Table 3).
On all variants of the experiment, mineral fertilizers were introduced. In the control without the use of chemical and biological preparations, the actual yield was 38.72 t/ha. Processing seed tubers before planting with Maxim fungicide (0.2 l/t) increased the yield of tubers by 1.01 t/ha. The largest increase in tuber yield, 4.61 t/ha, was obtained in option 7, where the preparation RECB-50 B (2 l/t) was used together with an adaptogen to treat tubers before planting, and during the growing season, the preparation was used for the first treatment of plants -RECB-95 B (0.5 l/ha) + adaptogen, for the second treatment -RECB-44 B (0.5 l/ha) + adaptogen, third -RECB -50 B (1.0 l/ha) + adaptogen. Table 3. The effect of chemical and biological preparations on the formation of potato tubers productivity, 2019 An analysis of the crop structure showed that the mass of tubers per bush in the early ripening variety Vineta, depending on the type of experiment, ranged from 936 to 1036 g/bush. In the structure of the crop, tubers of a large fraction mainly prevailed, their share in the crop was 65 %. The average fraction (40-80 g) of the studied variants for the studied variety was 28.3 %.  4 showed that a smaller amount of nitrates 73 mg/kg contained tubers with the option when used for processing tubers of strain RECB -50 B (2 l/t) and triple treatment of plants during the growing season with bioagents RECB -95 B (0.5 l/ha ), RECB -44 B (0.5 l/ha), RECB -50 B (1.0 l/ha). The use of these drugs in conjunction with the adaptogen led to an increase in the number of nitrates in the tubers. A similar picture was observed in other variants with the use of adaptogen. It should be noted that in all experimental variants the content of nitrates in tubers did not exceed the MAC. A higher starch content in tubers 13.02 and 13.01 % was observed when Maxim (0.2 l/t) and RECB strain -50 B (2 l/t) were used for treatment of tubers and triple treatment of plants during the growing season with RECB -95 B bioagents (0.5 l/ha), RECB -44 B (0.5 l/ha), RECB -50 B (1.0 l/ha). In the dry matter, the starch content was higher when the tubers were treated with rhizoplan and amounted to 63.38 %.

Conclusion
A higher yield at the level of 43.33 t/ha was formed when RECB strain -50 B (2 l/t) was applied for tuber treatment and triple treatment of plants during the growing season with the bioagents RECB -95 B (0.5 l/ha), RECB -44 B (0.5 l/ha), RECB -50 B (1.0 l/ha). In order to reduce the pesticidal load on the environment and improve the quality of tubers, this option should be used without adaptogen.