The chemical composition of the three-part beggarticks ( Bidens tripartita L.)

. In the present article, we have studied the main groups of biologically active substances of the bur-marigold ( Bidens tripartita L.) herb using the UPLC method with photodiode array and MS/MS detection. Eighteen compounds have been identified, including the glycosides of luteolin, okaniin, and sulfuretin, polyacetylenes, and hydroxycinnamic acids. The quantitative content of flavonoids (0.7-1.2%) and polysaccharides (4.1-7.5%) was computed by spectrophotometry and gravimetry.

B. tripartita herb is a well-studied PRM.Nevertheless, it seems appropriate to improve the quality indicators of the B. tripartita herb.
The qualitative and quantitative composition of BAS of plants can vary widely due to the presence of several chemotypes (chemical varieties), environmental conditions, and the composition of soils on which PRM are grown [17].In this regard, study the chemical composition of BAS in plants produced in a certain area is relevant.To improve the quality indicators, it is necessary to study the composition of the main groups of BAS in the B. tripartita herb cultivated on the territory of the Russian Federation.At present, there are no The purpose of this work is to study the composition and content of marker BAS of the B. tripartita herb, growing on the territory of the Russian Federation.One of the tasks of standardization is the search for highly specific marker compounds that serve to identify PRM.

Materials and methods
The objects of the study were samples of chopped B. tripartita herb.The gradient elution UPLC method with photodiode array and MS/MS detection was used (table 1).

Sample preparation Extraction with seventy percent methylic alcohol in an ultrasonic bath
To assess the content of polysaccharides (gravimetry, "polysaccharides" "PSC") and flavonoids (SP, "total flavonoids contents in terms of rutin"), the methods described in the PM SP RF XIV edition for the B. tripartita herb were used.A modified PSC method (SP, "total content of reducing sugars, in PSC") was also used (table 2) [18].

Discussion
In the course of the experiment, the main groups of BAS of the B. tripartita herb were identified and confirmed.The results received are conform with the literature information.
Of great interest is the polyacetylenes specific group (376, 378, 380, 418 and 420) (table 5).This is a rare BAS group in PRM.A common marker for plants of the B. tripartita genus is polyacetylene-418, which is found in all chemotypes.The complex of certain polyacetylenes makes it possible to carry out species identification of the B. tripartita.Polyacetylenes, along with the profile of flavonoids and hydroxycinnamic acids, are very important to use in the standardization of the B. tripartita herb.

Conclusion
An information-analytical study of the chemical composition of the B. tripartita herb was performed.As a result of the analysis, it was found that the main groups of BAS in the B. tripartita herb were polyacetylenes, flavonoids (okaniin and sulfuretin glycosides) and hydroxycinnamic acids.They are the main marker compounds that serve to identify PRM.It is also preferable to use the method of spectrophotometry instead of gravimetry, due to the greater rapidity of the method.
Conferences CIBTA-II-2023 https://doi.org/10.1051/bioconf/20237102005005 71 sufficiently complete and structured data on the component composition of samples of the B. tripartita herb of Russian origin.

Table 2 .
Methods for assessing the content of polysaccharides and flavonoids.

Table 4 .
Results of quantitative determination of polysaccharides and flavonoids.