Simultaneous determination of wine sugars, glycerol and organic acids 13C/12C isotopic ratio by ion chromatography-co-IRMS
Service Commun des Laboratoires, 3 Av. du Dr. A. Schweitzer; 33608 Pessac, France
Ion chromatography (IC) isotope ratio mass spectrometry (IRMS) coupling is possible using a liquid interface allowing chemical oxidation (co) of organic compounds. Synthetic solutions containing a mixture of glycerol, sugars (sucrose, glucose and fructose) and organic acids (gluconic, lactic, malic, tartaric, oxalic, fumaric, citric and isocitric) were elaborated to estimate analytical applications of two different IC systems. It appears that the use of carbonated solution in the eluting phase is a perturbation for 13C isotope measurements as it creates a δ13C deviation from the expected values. A sample of authentic sweet wine was analyzed by IC-co-IRMS using KOH gradient. Ratios of isotopic, ratios of organic acids and glycerol was found to be, in average, equal 1.01 ± 0.04 that is in accordance with our previous results.
© Owned by the authors, published by EDP Sciences, 2014
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