| Issue |
BIO Web Conf.
Volume 8, 2017
2016 International Conference on Medicine Sciences and Bioengineering (ICMSB2016)
|
|
|---|---|---|
| Article Number | 01019 | |
| Number of page(s) | 7 | |
| Section | Session I: Medicine | |
| DOI | https://doi.org/10.1051/bioconf/20170801019 | |
| Published online | 11 January 2017 | |
Assessment of DNA interstrand crosslinks in NIH/3T3 cells induced by Chloroethylnitrosoureas
College of Life Sciences and Bioengineering, Beijing University of Technology, Beijing Key Laboratory of Environment and Viral Oncology, Beijing, China
a Corresponding author: Chen xuechai. Email: chenxuechai@bjut.edu.cn
Aim: To investigated the mechanism of Chloroethylnitrosoureas (CENUs) leading to secondary tumors after therapy, three kinds of CENUs, namely, Nimustine (ACNU), Carmustine (BCNU) and Semustine (MeCCNU) were used to investigated drug-induced DNA interstrand crosslinks(ICLs). Method: The alkaline comet assay was adopted to compare DNA damages induced by CENUs under different concentrations. Results: With the increase of drug’s concentration, DNA migration exhibited a positive concentration-dependent relationship in all three drugs. ACNU was shown to cause significant crosslinking. The other two drugs also induced crosslinking, but this could not be analyzed at high concentrations due to the high cytotoxicity of the drugs which caused cells death.
The authors thank the support from China Postdoctoral Science Foundation (No. 2012M510302), and partial support from the Beijing Key Laboratory of Environment and Viral Oncology of China (No. PXM2012_014204_00_000181) and Youth Fund of the National Natural Science Foundation of China (NO. 81400935). The authors also acknowledge the support provided by the professors in the College of Life Sciences and Bioengineering, Beijing University of Technology and all colleagues in the laboratory.
© The Authors, published by EDP Sciences, 2017
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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