| Issue |
BIO Web Conf.
Volume 71, 2023
II International Conference on Current Issues of Breeding, Technology and Processing of Agricultural Crops, and Environment (CIBTA-II-2023)
|
|
|---|---|---|
| Article Number | 01076 | |
| Number of page(s) | 6 | |
| Section | Issues of Sustainable Development of Agriculture | |
| DOI | https://doi.org/10.1051/bioconf/20237101076 | |
| Published online | 07 November 2023 | |
Real-time multiplex PCR method for detection of A. veronii A. caviae A. salmonicida
FSBEI HE Ulyanovsk state agrarian university named after P. А. Stolypin, 1, Novy Venetz boulevard, Ulyanovsk, 432017, Russia
As a result of the experiments, the parameters for setting up a polymerase chain reaction were developed for the accelerated identification of Aeromonas spp. The design of species-specific primers was performed, the reaction parameters were selected, the specificity was checked, and the optimal amplification time was selected. As a result of this study, a multiplex real-time PCR test system was developed for the simultaneous detection of A. salmonicida, A. caviae and A. veronii. The sensitivity of the developed protocol was 5.56 pg/µl. The data obtained will allow for accelerated screening of these pathogens.
© The Authors, published by EDP Sciences, 2023
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