Effect of Indigofera sp. Diet Towards Blood Serum Biochemistry of Indonesian Crossbred Native Chickens

¹ Institute for Agricultural Instrument Standard Implementation-Bali, Ngurah Rai Rd., Pesanggaran, South Denpasar-Denpasar, Bali 80222, Indonesia
² Research Center for Animal Husbandry, Research Organization for Agriculture and Food, National Research and Innovation Agency, Bali 80223, Indonesia
³ Institute for Agricultural Instrument Standard Implementation-Yogyakarta, Maguwoharjo Stadium Rd, Sleman, Yogyakarta 55584, Indonesia

^* Corresponding author: dharmawan29.id@gmail.com

Abstract

A study was conducted to assess the effects of Indigofera sp. meal on the serum biochemistry of Kampung Unggul Balitbangtan (KUB) chickens, a native strain released by the Indonesian Minister of Agriculture via Decree No. 274/Kpts/SR.120/02/2014. Forty KUB chickens, aged 8-16 weeks, were raised in floor cages with ad libitum access to water. Four dietary treatments were administered: T0 (control: 25% concentrate, 35% rice bran, 40% maize); T1 (15% concentrate, 35% rice bran, 10% Indigofera sp. meal, 40% maize); T2 (T1 + 2cc/l Bio-B); and T3 (22% concentrate, 31.5% rice bran, 10% Indigofera sp. meal, 36.5% maize). Serum samples, obtained via jugular venipuncture and centrifugation, were analyzed for blood urea nitrogen (BUN), total protein, total cholesterol, and glucose. A randomized block design was employed, and data were analyzed using Duncan's post hoc test (SPSS 26). Significant differences (p<0.05) in BUN and total cholesterol were observed in the T1 group compared to the control, while total protein and glucose levels remained unaffected. The findings suggest that the T1 diet is suitable for sustainable KUB chicken production.