| Issue |
BIO Web Conf.
Volume 231, 2026
International Scientific Conference “Fundamental and Applied Scientific Research in the Development of Agriculture in the Far East and Remote Regions: Transforming Agri-Systems through Disruptive Innovation” (AFE-2025)
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|---|---|---|
| Article Number | 00039 | |
| Number of page(s) | 10 | |
| DOI | https://doi.org/10.1051/bioconf/202623100039 | |
| Published online | 10 April 2026 | |
Pathogenicity Evaluation of Fungal Isolates on Crimean Pine (Pinus nigra subsp. pallasiana) Seeds and Seedlings
1 Tashkent State Agrarian University, 100140 Kibray District, Tashkent Region, Uzbekistan
2 Plant Quarantine and Protection Research Institute, 111215 Kibray District, Tashkent Region, Uzbekistan
* Corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it.
Abstract
This study presents data on the evaluation of the pathogenicity of fungal isolates causing damping-off disease in seeds and seedlings of Crimean pine (Pinus nigra subsp. pallasiana) and their identification based on morphological and molecular methods. A total of eight fungal isolates were obtained from diseased seedlings, of which four were selected for further investigation. The results of pathogenicity tests demonstrated differences in virulence among the isolates, with Fusarium brachygibbosum No. 32 and Fusarium proliferatum No. 36 showing the highest pathogenicity at both seed and seedling stages. Koch's postulates were fully satisfied for these isolates. Molecular-genetic analysis based on the TEF-1α gene identified isolate No. 32 as Fusarium brachygibbosum and isolate No. 36 as Fusarium proliferatum. The results indicate that F. brachygibbosum is reported for the first time as a causal agent of damping-off in Crimean pine. In addition, F. proliferatum also exhibited high pathogenic potential. The findings suggest that damping-off disease may be caused by multiple Fusarium species and highlight the importance of accurate pathogen identification and the application of integrated phytosanitary measures for the production of healthy seedlings.
© The Authors, published by EDP Sciences, 2026
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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