Open Access


The general characteristics of the ISSR primers used in the somaclonal variation tests of Fritillaria dagana

Primer sequence (5'-3') Number of bands, annealing temperature (°C) Band size, bp
51 °C 56 °C
(CT)8TG 8 9 500-1500
(CAC)3GC 14 15 200-2500
(CTC)3GC 14 17 200-2300
(CA)6GT 12 15 200-2500
(CA)6AG 8 14 250-2000
(AG)8YC* 8 14 450-2000

* Y = C or T

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