Open Access
Table 1
Characteristics of ISSR primers tested and selected to study rose genetic polymorphism
primers | Sequence, 5’-3’ | C° | TNB* | NBP* | PPB*, % |
---|---|---|---|---|---|
HB 12 | (CAC)3 GC | 42°C | 26 | 23 | 88,46 |
17899 A | (CA)6 AG | 49°C | 21 | 20 | 95,24 |
17899 B | (CA)6 GG | 42°C | 23 | 22 | 95,65 |
UBC 807 | (AG)8 T | 52°C | 22 | 21 | 95,45 |
UBC 834 | (AG)8 YT | 60°C | 19 | 18 | 94,74 |
UBC 855 | (AC)8 YT | 50°C | 25 | 23 | 92,0 |
M2 | (AC)8 YG | 50°C | 20 | 19 | 95,0 |
M9 | (GACAC)4 | 50°C | 19 | 18 | 94,74 |
* TNB – total number of bands, NPB – number of polymorphic bands, PPB – percentage of polymorphic bands, %
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