Table 1
The effect of the growth regulator of 6-benzylaminopurine and 2,4-dichlorophenoxyacetic acid on the morphogenesis of R chamaemorus microspores (MS nutrient medium, sucrose 30 g/l, pH 3.8; microspore density 80000 in 0.5 ml, “-” explant death)
The growth regulator concentration, mg/l | The appearance of embryoids, days | The number of embryoids formed on the 60th day, pcs. | ||
---|---|---|---|---|
M±SEM1,2 | Cv, % | M±SEM1,2 | Cv, % | |
Sort Nubu | ||||
2,4-dichlorophenoxyacetic acid | ||||
0.1 | 67±2a | 4.8 | 16±1 | 3.2 |
0.2 | 59±1 | 4.9 | 33±2ab | 7.3 |
0.3 | 69±2ab | 9.0 | 25±2ab | 5.4 |
0.4 | 72±4abc | 3.2 | 9±3 | 2.3 |
6-benzylaminopurine | ||||
0.5 | - | - | - | - |
1.0 | - | - | - | - |
1.5 | 74±2a | 4.7 | 8±2 a | 4.5 |
2.0 | 75±3ab | 5.2 | 7±3ab | 8.2 |
Male form from a natural population | ||||
2,4-dichlorophenoxyacetic acid | ||||
0.1 | 63±3 | 9.1 | 15±3 | 4.4 |
0.2 | 50±1 | 10.2 | 52±5 | 7.3 |
0.3 | 66±1 | 6.3 | 32±1 | 5.1 |
0.4 | - | - | - | - |
6-benzylaminopurine | ||||
0.5 | - | - | - | - |
1.0 | 87±12 a | 17.2 | 4±1 a | 9.5 |
1.5 | 89±9 a | 13.5 | 8±2 a | 5.7 |
2.0 | - | - | - | - |
Note. Each experiment was performed in triplicate. Dashes mean the death of explants. 1 – confidence interval based on student t-distribution at a significance level of 0.05; 2 – the values in the column marked with the same lowercase letters (a, b, c) do not have a significant difference at the 5% significance level (P≤0.05) according to the t-student test
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