Table 2
The effect of the concentration of the mother liquor of nutrient media MS and WPM on the development of R chamaemorus embryoids (growth regulator 2,4-dichlorophenoxyacetic acid, sucrose 30 g/l, pH 3.8; microspore density 80000 in 0.5 ml, “-” the death of explants)
| The concentration of the base solution,% | The beginning of embryoid growth*, day | Total length after 40 days of cultivation, mm | ||
|---|---|---|---|---|
| M±SEM1,2 | Cv, % | M±SEM1,2 | Cv, % | |
| sort Nubu | ||||
| MS | 47±1 a | 4.8 | 2.2±1.5 a | 3.2 |
| 2/3 MS | 34±2 | 4.9 | 5.5±1.0 | 7.3 |
| 1/2MS | 45±1 a | 9.0 | 3.1±1.0 a | 5.4 |
| Male form from a natural population | ||||
| WPM | 67±1.0 a | 9.1 | 1.1±1.0 a | 14.4 |
| 2/3 WPM | 66±1.0 ab | 10.2 | 1.2±0.8 ab | 17.3 |
| 1/2 WPM | 70±1.0 ab | 6.3 | 0.9±0.3 ab | 15.1 |
Note. Each experiment was performed in triplicate. Dashes mean the death of explants. 1 – confidence interval based on student t-distribution at a significance level of 0.05; 2 – the values in the column marked with the same lowercase letters (a, b) do not have a significant difference at the 5% significance level (P≤0.05) according to the t-student test
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