| Issue |
BIO Web Conf.
Volume 218, 2026
The 12th International Conference of Innovation in Animal Science: “Animal Agriculture and the SDGs: Balancing Productivity, Welfare, and Environmental Integrity (ICIAS 2025)
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|---|---|---|
| Article Number | 05001 | |
| Number of page(s) | 8 | |
| Section | Animal Reproduction and Breeding | |
| DOI | https://doi.org/10.1051/bioconf/202621805001 | |
| Published online | 10 February 2026 | |
Quality and Proportion of Friesian Holstein Bull Sperm Using Egg White Albumin Sedimentation Sexing Method with Different Gradients
1 Graduate Student, Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia.
2 Undergraduate Student, Department of Animal Science, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia
3 Singosari National Artificial Insemination Center, Malang, 65153, Indonesia
4 Faculty of Animal Science, Universitas Brawijaya, Malang, 65145, Indonesia
5 Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
6 Institute of Tropical Agriculture and Food Security (ITAFoS), Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
* Corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it.
Abstract
This study aims to evaluate the effect of different gradients on the quality and proportion of sexed semen using the egg white albumin sedimentation method. This study was conducted at the Singosari Artificial Insemination Center in Malang, using Friesian Holstein semen with an average fresh motility of >70%. The study was conducted experimentally with 10 replicates and 2 treatments, namely T1 = egg white sedimentation sexing with 2 gradients (30% and 50%) and T2 = egg white sedimentation sexing with 3 gradients (30%, 40%, and 50%). The parameters observed included motility, viability, abnormality, membrane integrity, and the proportion of X and Y chromosomes. The quality evaluation results were analyzed using an independent t-test, while the determination of the spermatozoa proportion was analyzed using the Chi-Square test. The results showed that differences in gradients did not have a significant effect (p < 0.05) on any quality parameters in each layer. In addition, the proportion of X and Y spermatozoa also showed no difference between the upper and lower fractions in both layers (p > 0.05). In conclusion, spermatozoa separation using 3-gradient egg white albumin sedimentation tended to provide more efficient separation while maintaining spermatozoa quality, although no significant differences were observed compared to the 2-gradient method.
Key words: Egg White Albumin / Friesian Holstein Bull / Gradient-Based Separation / Semen Quality / Sperm Sexing
© The Authors, published by EDP Sciences, 2026
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