| Issue |
BIO Web of Conferences
Volume 6, 2016
Electro-Activity of Biological Systems
|
|
|---|---|---|
| Article Number | 01003 | |
| Number of page(s) | 1 | |
| Section | Subcellular systems | |
| DOI | https://doi.org/10.1051/bioconf/20160601003 | |
| Published online | 22 March 2016 | |
A direct and at nanometer scale study of electrical charge distribution on membranes of alive cells
Institut des Sciences Moléculaires d’Orsay (ISMO), Université Paris-Sud, Université Paris-Saclay, 91405 Orsay Cedex, France
a Corresponding author: christian.marliere@u-psud.fr
In this paper is presented an innovative method to map in-vivo and at nanometer scale the electrical charge distribution on membranes of alive cells. It relies on a new atomic force microscopy (AFM) mode based on an electro-mechanical coupling effect. Furthermore, an additional electrical signal detected by both the deflection of the AFM cantilever and simultaneous direct current measurements was detected at low scanning rates. It was attributed to the detection of the current stemming from ionic channels. It opens a new way to directly investigate in situ biological electrical surface processes involved in bacterial adhesion, biofilm formation, microbial fuel cells, etc.
© Owned by the authors, published by EDP Sciences, 2016
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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