| Issue |
BIO Web Conf.
Volume 8, 2017
2016 International Conference on Medicine Sciences and Bioengineering (ICMSB2016)
|
|
|---|---|---|
| Article Number | 01017 | |
| Number of page(s) | 5 | |
| Section | Session I: Medicine | |
| DOI | https://doi.org/10.1051/bioconf/20170801017 | |
| Published online | 11 January 2017 | |
Fluorescence spectral studies on interaction of SEGS with BSA
1 Laboratory of molecular biology for TCM, Henan University of Chinese Medicine, Zhengzhou 450046, China
2 National Key Laboratory of Cryospheric Sciences/Tien Shan Glaciological Station, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China
a Corresponding author: contributepaper@126.com
The seeds of Gleditsia sinensis is one of the most effective drugs for the treatment of esophageal carcinoma. To definite the active ingredients of the seeds of gleditsia sinensis, SEGS was extracted from the seeds of Gleditsia sinensis. Investigations the interaction between the active ingredient of traditional Chinese medicine and BSA is helpful for explaining the medicine mechanism. Under the simulative human physiological conditions, the interaction between SEGS and bovine serum albumin (BSA) was investigated by fluorescence spectroscopy. The quenching mechanism of SEGS with BSA is dynamic quenching, SEGS bound with BSA by van der Waals forces or hydrogen bond. The binding constants and the number of binding sites between SEGS and BSA at different temperatures (298 and 308 K) were obtained.
This research was supported by the special grade of the financial support from the China Postdoctoral Science Foundation (Grant No.2016T90669), the National Science Foundation for Post-doctoral Scientists of China (Grant No.2015M582189), the National Science Foundation (Grant No.81550014), the Henan Postdoctoral Sustentation Fund, China (Grant No. 00104256), Jinshui District Science and Technology Bureau Social Program (Grant No. 20132602).
© The Authors, published by EDP Sciences, 2017
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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