BIO Web Conf.
Volume 18, 2020IV All-Russian Plant Protection Congress with international participation “Phytosanitary Technologies in Ensuring Independence and Competitiveness of the Agricultural Sector of Russia”
|Number of page(s)||3|
|Published online||06 March 2020|
Vairimorpha (Nosema) ceranae (Opisthosporidia: Microsporidia) in vitro Infection of Sf9 Insect Cell Line as an Experimental Model of Parasite – Host Interrelations
All-Russian Institute of Plant Protection, Podbelskogo 3, 196608, St. Petersburg, Pushkin, Russia
* Corresponding author: email@example.com
Microsporidia Vairimorpha (Nosema) ceranae is an emergent parasite of honeybee Apis mellifera and has a great concern to apiculture due to high pathogenesity and fumagillin resistance. Recently RNA interference and single-chain antibodies technologies are proposed to create unsusceptible to intracellular parasites breeds of insects. In this study we established cell culture model system to provide a way to investigate the molecular basis of interactions between honeybee and its pathogen. As the first step in this direction V. ceranae spores were isolated from living bees, properly sterilized and in vitro germinated with Sf9 lepidopteran-derived cell line. The entire life circle of this parasite was achieved. This should allow us to develop the novel treatment strategy directed to inhibit propagation of V. ceranae intracellular stages with recombinant antibodies targeted to the vitally important membrane transporters.
© The Authors, published by EDP Sciences, 2020
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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