Anatomical Structure and Terpenoid Content of Zodia (Evodia suaveolens Scheff) Leaves

Biology Department, Faculty of Mathematics and Natural Sciences, Universitas Lambung Mangkurat. Jl. A. Yani Km 36 Banjarbaru, South Kalimantan, Indonesia

^* Corresponding author: evimintowati@ulm.ac.id

Abstract

Zodia (Evodia suaveolens Scheff) is a member of Rutaceae contain terpenoids, triterpenoids, alkaloids, flavonoids, and xanthones which have anti-mosquito activity. This research aimed to observe the anatomical structure, the location, and distribution of terpenoid based on the leaves' age. Anatomical slides preparation of leaves were made using the paraffin embedding method with safranin staining. The distribution of terpenoid was analyzed by the histochemical assay. Leaf anatomical structure shows that the 3^rd and 6^th leaf bifacial (dorsiventral) consisted of the upper epidermis, mesophyll (palisade and sponge), collateral vascular bundle, parenchyma midrib, abaxial epidermis and oil glands in mesophyll that is underneath both epidermises. The diameter of oil glands with larger sizes was on the 6^th leaf, whereas the density is not different in the 3^rd and 6^th leaves. The histochemical test showed that terpenoid was observed in the leaf vascular bundles, oil glands, and epidermis.