Proapoptosis Effect of Root of Eurycoma longifolia (Pasak Bumi) on the Prostate Cancer: In Silico Analysis

¹ Doctoral Program of Medical Science, Faculty of Medicine, University of Brawijaya Malang, Indonesia
² Department of Surgery, Urology Division, Faculty of Medicine, University of Lambung Mangkurat Banjarmasin, Indonesia
³ Department of Pharmacology, Faculty of Medicine, University of Brawijaya Malang, Indonesia
⁴ Department of Urology, Faculty of Medicine, University of Brawijaya Malang, Indonesia
⁵ Department of Anatomical Pathology, Urology Division, Faculty of Medicine, University of Lambung Mangkurat Banjarmasin, Indonesia

^* Corresponding author: eyurologi05@gmail.com

Abstract

This study aimed to predict the proapoptosis effect of E. longifolia active compounds on prostate cancer by in silico analysis. Protein data such as BCL-2 (GI: 2506216), Caspase 3 (GI: 6978605), Caspase 8(GI: 11560103), data quassinoid (ID: 5459060 and chantin (ID: 97176) were collected from GenBank of NCBI. Protein BCL-2 collected from NCBI compare with Protein Data Bank (PDB) and UNIPROT. The docking process was carried out using software HEX 8.0. to compute the binding affinity between ligands (active compounds of Pasak Bumi) and protein target. The interaction between quassinoid and chantin was strongest and stable against caspase-9, indicating that the active ingredient in E. longifolia triggered caspase-9 activity after activation of BH3 domains in Bcl-2 in prostate cancer. The low energy binding between quassinoid and chantin with caspase-3 indicates the interaction between the active ingredients is not strong with caspase-3. E. longifolia active ingredients that are potentially used in the treatment of prostate cancer are quassinoid and chantin by inducing apoptotic mechanisms via both extrinsic and intrinsic pathways. The combination of active ingredients of E. longifolia that is quassinoid and chantin can be used as a strategy of prostate cancer therapy both through extrinsic and intrinsic pathways.