BIO Web Conf.
Volume 21, 2020XI International Scientific and Practical Conference “Biological Plant Protection is the Basis of Agroecosystems Stabilization”
|Number of page(s)||6|
|Published online||22 June 2020|
Secondary screening of strains of antagonists to a Phoma pathogen on sunflower
1 V.S. Pustovoit All-Russian Research Institute of Oil Crops, 17, Filatova str., Krasnodar, 350038, Russia
2 Kuban State University, 149, Stavropolskaya str., Krasnodar, 350040, Russia
* Corresponding author: email@example.com
There are presented data of the first stage of the secondary screening of antagonist strains from a collection of the biological methods laboratory of V.S. Pustovoit All-Russian Research Institute of Oil Crops to the aggressive isolate of Phoma macdonaldii Boerema infecting sunflower. We used methods of ‘agar blocks’ and ‘perforated Petri dishes’. As a result of a screening of 55 antagonist strains to Phoma macdonaldii pathogen we selected 17 promising strains-producers of microbiological preparations having biological efficiency exceeding 40.0 %. In control variant without seed treatment sunflower seedlings infection was up to 76.2 %, and maximal biological efficiency of laboratory samples from fungi-producers were determined for a strain M-24 Penicillium sp. (73.8 %); from bacteria of a genus Bacillus – for a strain D-10 Bacillus sp. (74.2%); from bacteria of a genus Pseudomonas – for a strain Sgc-1 Pseudomonas sp. (73.8 %). Maximal colonizing activity were noted for fungi-producers M-24 Penicillium sp. (85.7 %) and Xk-1 Ch. olivaceum (71.4 %), as well as for bacteria of a genus Bacillus – 5B-1 B. subtilis (71.4 %) and a genus Pseudomonas – Oif 2-1 Pseudomonas sp. and 14-3 P. chlororaphis (71.4 %) against Phoma infection in control of 70.0 %.
© The Authors, published by EDP Sciences, 2020
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