BIO Web Conf.
Volume 22, 2020International Conference “Longevity Interventions 2020” (ICLI 2020)
|Number of page(s)||9|
|Section||Actual Problems of Medicine|
|Published online||06 July 2020|
Mitochondrial activity of cancer and normal mesenchymal stem cells in vitro cultured in medium with different deuterium content
Peoples Friendship University of Russia (RUDN University), 117198, Miklukho-Maklaya Str., 6, Moscow, Russia
2 State Institute of Genetic and Regenerative Medicine of NAMS of Ukraine, 04114, Vyshhorodska Str., 67, Kiev, Ukraine
3 Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, RAS, 117997, Miklukho-Maklaya Str., 16/10, Moscow, Russia
4 Biotechnology Laboratory ilaya regeneration, medical company ilaya, 03115, Kiev, Ukraine
5 Institute of Colloid and Water Chemistry, NAS of Ukraine, 03142, Akademika Vernadskoho Boulevard, 42, Kyiv, Ukraine
* Corresponding author: firstname.lastname@example.org
We showed that cancer and normal mesenchymal stem/stromal (MSC) cells in vitro in a deuterated growth medium show a decrease of mitochondrial activity (MA), while in a deuterium-depleted medium an increase. This was established using mitotracker and rhodamine 123, and was also confirmed by expression of the UCP1 gene. The correlation dependence of mitochondrial activity in a medium with a changed ratio of deuterium/protium (D/H) and supramolecular structures was established, using the laser diffraction method. Density inhomogeneities in the deuterated medium are noted to be large, and in the deuterium-depleted medium small, in comparison with the control.
© The Authors, published by EDP Sciences, 2020
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