BIO Web Conf.
Volume 23, 2020II International Scientific Conference “Plants and Microbes: The Future of Biotechnology” (PLAMIC2020)
|Number of page(s)||8|
|Published online||14 August 2020|
Optimization of core phases of biolistic transformation of potato
1 M. A Aitkhozhin Institute of Molecular Biology and Biochemistry, Laboratory of Plant Bioengineering and Laboratory of Genome, 050012 Almaty, Republic of Kazakhstan
2 Almaty Branch of National Center for Biotechnology in Central Reference Laboratory (CRL), 050054 Almaty, Republic of Kazakhstan
3 Institute of Plant Biology and Biotechnology, 050040 Almaty, Republic of Kazakhstan
* Corresponding author: firstname.lastname@example.org
We have established the optimal exposure time for internodes and callus plant material of Aksor potato variety on the OSS osmotic medium before and after biolistics and the type of plant explant that is most suitable for that. The optimal objects were explants from internodes of test plants. The best time for exposure of plant material to the osmotic medium before bombardment is 24 hours, and after biolistics – 48 hours. Callus tissue developed on the MS medium with zeatin two weeks later in internodes that were cultivated before bombarding on the OSS for 24 hours and we observed an increase in the callus mass by the end of fourth week. Not a single callus was obtained with rhizogenesis on the R4 medium from internodes and potato calli that were on the osmotic medium within 24 and 72 hours after biolistics. The regenerative capacity of internodes after cultivation on an osmotic medium for 48 hours after bombarding was higher than that of calli. We obtained 12 regenerant plants out of 200 internode explants and 4 regenerant plants out of 150 calli that were used in the experiment.
© The Authors, published by EDP Sciences, 2020
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