Structural lipids and carbohydrates of the deep mycelium of phoma-like micromycetes, potential mycoherbicides

¹ All-Russian Institute of Plant Protection, Laboratory of Toxicology and Biotechnology, 196608 Saint Petersburg, Russia
² Saint-Petersburg State Institute of Technology, Department of Molecular Biotechnology, 190013 Saint Petersburg, Russia
³ Saint Petersburg State University, Research park, Centre for Molecular and Cell Technologies, 199034 Saint Petersburg, Russia

^* Corresponding author: svsokornovs@vizr.spb.ru

Abstract

The work is devoted to the mycelium biochemical composition of Stagonospora cirsii C-211, Calophoma complanata 32.121, Didymella macrostoma 32.52. These phylogenetically distant species of phoma-like micromycetes are the potential mycoherbicides of Cirsium arvense, Heracleum sosnowskyi, and Convolvulus arvensis, respectively. The S. cirsii C-211, C. complanata 32.121, D. macrostoma 32.52 mycelium in the early stationary growth phase was obtained on sucrose-soybean nutrient medium. It was shown that the lipid and carbohydrate (polyols, sugars) profiles of these strains have much in common. We suppose that levels of arabitol and trehalose influence to the stress-resistant of phoma-like micromycetes. In particularly, these carbohydrates serve structural and protective roles in the cell walls during osmotic and temperatures stress. The ratio of phosphatidylcholine to phosphatidylethanolamine and the proportion of phosphatidylserine among structural lipids also determine the properties of mycelium, and can be used to assess its quality.