| Issue |
BIO Web Conf.
Volume 24, 2020
International Conferences “Plant Diversity: Status, Trends, Conservation Concept” 2020
|
|
|---|---|---|
| Article Number | 00021 | |
| Number of page(s) | 5 | |
| DOI | https://doi.org/10.1051/bioconf/20202400021 | |
| Published online | 21 September 2020 | |
In vitro propagation of endemic species Hedysarum chaiyrakanicum (Tuva Republic, Russia) and its widespread congener, H. gmelini (Fabaceae)
CSBG SB RAS, 630090, 101, Zolotodolinskaya st., Novosibirsk, Russia
* Corresponding author: annaerst@yandex.ru
A rare species Hedysarum chaiyrakanicum and a highly polymorphic species H. gmelini were first introduced into in vitro culture. It was shown that MS supplemented by 5 µM BAP is optimal medium for micropropagation of H. gmelini from 90-1 and 92-1 subpopulations, medium with 1 µM BAP is optimal for micropropagation of H. chaiyrakanicum from 45-2 subpopulation, and 10 µM BAP – for H. chaiyrakanicum from 45-1 subpopulation. Seedlings obtained from the seeds of H. gmelinii collected from the subpopulation no. 88 demonstrated a higher tendency to callus formation using BAP. It was also found that in vitro culture of H. chaiyrakanikum was characterized by a higher reproduction rate then H. gmelinii in vitro culture.
© The Authors, published by EDP Sciences, 2020
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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