| Issue |
BIO Web Conf.
Volume 24, 2020
International Conferences “Plant Diversity: Status, Trends, Conservation Concept” 2020
|
|
|---|---|---|
| Article Number | 00043 | |
| Number of page(s) | 6 | |
| DOI | https://doi.org/10.1051/bioconf/20202400043 | |
| Published online | 21 September 2020 | |
Features of development of hametophytes Botrychium multifidum (S.G. Gmel.) Rupr. culture in vitro
1 Kostroma State University, 156000, 14, 1st of May St., Kostroma, Russia
2 7631818356, Kerman, Graduate University of Advanced Technology, Iran
3 Volga State University of Technology, 424000, 3. Lenin Sq., Yoshkar-Ola, the Republic of Mari El, Russia
* Corresponding author: zontikovdn@mail.ru
The work is devoted to studying the influence of the type of culture medium and pH on the development of gametophytes Botrychium multifidum in vitro. The spores obtained from sterilized sporangia were suspended in liquid nutrient media with initial inoculum of 10000 spores per 1 ml. Nutrient media tested in the study were composed of full Murashige and Skoog or Knudson mineral salts supplemented with kinetin (1 mg/l) and pH level 4.8-6.4. All stages of development, from spore germination to thallus and gametophyte formation, were observed in tissue culture. A low level of germinating spores was noted.
© The Authors, published by EDP Sciences, 2020
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