| Issue |
BIO Web Conf.
Volume 25, 2020
International Scientific Online-Conference “Bioengineering in the Organization of Processes Concerning Breeding and Reproduction of Perennial Crops” 2020
|
|
|---|---|---|
| Article Number | 04001 | |
| Number of page(s) | 5 | |
| Section | Genetic Engineering in vitro in Breeding, Exploration and Development of the Genetic Resources | |
| DOI | https://doi.org/10.1051/bioconf/20202504001 | |
| Published online | 01 October 2020 | |
Biotechnological methods of preservation of the grape gene pool in the in vitro collection
All-Russian Research Institute for Viticulture and Winemaking named after Ya.I. Potapenko – Branch of Federal State Budget Scientific Institution «Federal Rostov Agricultural Research Center», 166 Baklanovsky, Novocherkassk, Rostov region, 346421 Russia
The article shows the results of the improvement of meristem survival, its growth characteristics, shoot formation with the combined usage of apical meristems and the preparations such as Ribavirin, salicylic acid, Melafen, the antibiotic Cefotaxime. The research results demonstrate a decrease in the intensity of growth with the presence of sucrose, fructose, and sorbitol in the culture medium. The clarification of the parameters of the preparations application made it possible to increase the duration of nonstop storage of plants up to 8–10 months.
© The Authors, published by EDP Sciences, 2020
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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