BIO Web Conf.
Volume 25, 2020International Scientific Online-Conference “Bioengineering in the Organization of Processes Concerning Breeding and Reproduction of Perennial Crops” 2020
|Number of page(s)||6|
|Section||Getting Healthy Planting Material of Horticultural Crops and Grape Varieties by Means of Bioengineering|
|Published online||01 October 2020|
Micropropagation of rootstocks of stone fruit cultures in vitro
Federal State Budget Scientific Institution «North Caucasian Federal Scientific Center of Horticulture, Viticulture, Wine-making», 39 str. 40 Let Pobedy, Krasnodar, 350901, Russia
* Corresponding author: firstname.lastname@example.org
The usage of the method of clonal micropropagation of plants is currently the most promising for obtaining virus-free plant material, including clonal rootstocks of stone fruit cultures. The introduction of clonal rootstocks of stone fruit cultures PK SK1, PK SK 2, AI 1 into invitro culture is best doneduring the active shoot growth period in the second decadeof May. During this period, the highest level of explants regeneration was noted: in the rootstock PK SK 1 – 94,6 %, in the rootstock AI 1 78.2%, in PK SK 2 80.4%, in Gisela 5 (control) 85,7%. From the third decade of May, the survival rate of explants begins to decline. At the stage of multiplication on the Murashige-Skoog medium, with the addition of 6-BAP 1 mg/L, the average multiplication factor of clonal rootstocks to the fourth passage was 1: 12 for PK SK1, for Gisela 5 and for AI 11: 8.
© The Authors, published by EDP Sciences, 2020
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