BIO Web Conf.
Volume 25, 2020International Scientific Online-Conference “Bioengineering in the Organization of Processes Concerning Breeding and Reproduction of Perennial Crops” 2020
|Number of page(s)||7|
|Section||Modern Methods of Diagnosis and Monitoring of Phythopathogene and the Usage of Such Methods in Problem Solution in Plant Nursery|
|Published online||01 October 2020|
Phytoplasma disease “Bois noir” in Crimea: diagnosis of the pathogen
FSBSI All-Russian National Research Institute of Phytopathology, 5-а Institute str., 143050 Bolshiye Vyazyomy work settlement, Odintsovskiy district, Moscow region, Russia
2 FSBSI All-Russian National Research Institute of Viticulture and Winemaking Magarach of the RAS, 31 Kirova str., 298600 Yalta, Republic of Crimea, Russia
* Corresponding author: firstname.lastname@example.org
In 2018-2019, grape leaf samples with symptoms of yellowing (reddening) and leaf rolling collected from grapevines of different regions of Crimea were tested for the presence of phytoplasma using the “nested”PCR with primer pairs P1/16S-Sr and R16F2n/R16R2. Phytoplasmas were detected in 91% grape samples of 2018 and 46% samples of 2019. Genotyping performed by restriction fragment length polymorphism analysis for three genes: ribosomal (16S rRNA), the tuf gene encoding the elongation factor EF-Tu, and the vmp1 gene encoding a membrane protein, showed that the phytoplasmas isolated from all samples were related to the species Candidatus Phytoplasma solani, type “b”. Polymorphism was observed only for the vmp1 gene. The electrophoretic profiles of grape phytoplasma of 2018 (‘Chardonnay’ variety) differed from those of grape phytoplasma of 2019 (‘Pinot Noir’ and ‘Verdelho’ varieties), as well as from the profiles published in well-known works, which indicates the genetic diversity.
© The Authors, published by EDP Sciences, 2020
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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