| Issue |
BIO Web Conf.
Volume 28, 2020
The 3rd International Conference on Bioinformatics, Biotechnology, and Biomedical Engineering (BioMIC 2020)
|
|
|---|---|---|
| Article Number | 02001 | |
| Number of page(s) | 4 | |
| Section | Biomedical Sciences and Engineering | |
| DOI | https://doi.org/10.1051/bioconf/20202802001 | |
| Published online | 17 December 2020 | |
Amino acid identification of Bombyx mori fibroin cocoon as biomaterial using liquid chromatography/mass spectrometry
1 Department of Oral Biology, School of Dentistry, Universitas Muhammadiyah Yogyakarta, Yogyakarta, Indonesia
2 Department of Dental Biomaterial, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia
3 Department of Chemistry, Faculty of Mathematics and Natural Science Universitas Gadjah Mada, Yogyakarta, Indonesia
4 Department of Conservative Dentistry and Endodontic, Faculty of Dentistry Universitas Gadjah Mada, Yogyakarta, Indonesia
5 Department of Histology and Cells Biology, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
* Corresponding author: sartika.puspita@umy.ac.id
A fibroin is a natural polymer that serves as a biomaterial because of the mechanical properties, biocompatible, biodegradable and amino acid composition. The mechanical properties of fibroin are influenced by crystalline structures of amino acid and are related to the amino acid composition. it can accelerate the wound healing because it promotes the proliferation of exposure tissue and inhibits inflammation. The study aimed to identify amino acid of Bombyx mori (B. mori) fibroin cocoon as biomaterial that was cultivated in Central Java Indonesia. Liquid Chromatography/Mass Spectrometry (LC/MS-Water, USA) was employed. This study found that fibroin of B. mori contains seventeen amino acids and the most abundant amino acid is L-Valin 4.81 % (mg kg–1).
Key words: amino acid / Bombyx mori / dental material / fibroin / LCMS
© The Authors, published by EDP Sciences, 2020
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