BIO Web Conf.
Volume 33, 2021The 1st International Conference of Advanced Veterinary Science and Technologies for Sustainable Development (ICAVESS 2021)
|Number of page(s)||10|
|Section||Bioengineering and Biotechnology|
|Published online||23 August 2021|
Polymorphism of CSN1S1 (g.12164G>A) and CSN2 (g.8913C>A) genes in pure and cross dairy goats
Indonesian Research Institute for Animal Production (IRIAP), PO Box 221 Ciawi, Bogor, Indonesia
2 Faculty of Animal Science, Bogor Agriculture University, Bogor, Indonesia
* Corresponding author: email@example.com
Casein genes directly control milk protein of animals. CSN1S1 (αS1-Casein) and CSN2 (β-Casein) genes influence on milk protein fractions. Genetic polymorphisms of CSN1S1 gene at g.12164G>A locus and CSN2 gene at g.8913C>A locus were identified by PCR-RFLP technique. Animal samples were pure dairy goats providing PE (5 hds.), Saanen (8 hds.) and their crosses providing Sapera (50% Saanen, 50% PE) (51 hds.) and SaanPE (75% Saanen, 25% PE) (3 hds.) from IRIAP dairy goat station. Allele frequency, genotype frequency, heterozygosity value, and Hardy-Weinberg (H-W) equilibrium value were analyzed by Popgen32 program. CSN1S1_g.12164G>A locus resulted in two alleles, i.e. G allele (192 bp, 145 bp, and 101 bp) and A allele (337 bp and 101 bp). The G allele from the highest frequenciest was successively Saanen (0.625), Sapera (0.578), PE (0.400), and SaanPE (0.333). Most dairy goats were heterozygote (Ho>He) and in H-W equilibrium (q2 count < q2P0.05). Whereas CSN2_g.8913C>A locus was monomorphic for possesing only C allele (233 bp and 162 bp), without A allele (416 bp). The existent g.12164G>A SNP of the CSN1S1 gene of could be a potencial molecular selection marker of milk protein content in dairy goat.
Key words: Dairy goats / CSN1S1 gene / CSN2 gene / genetic polymorphism
© The Authors, published by EDP Sciences, 2021
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