| Issue |
BIO Web Conf.
Volume 33, 2021
The 1st International Conference of Advanced Veterinary Science and Technologies for Sustainable Development (ICAVESS 2021)
|
|
|---|---|---|
| Article Number | 03001 | |
| Number of page(s) | 5 | |
| Section | Advanced Veterinary Pharmacology for Sustainable Agriculture | |
| DOI | https://doi.org/10.1051/bioconf/20213303001 | |
| Published online | 23 August 2021 | |
The cytotoxic activity of pine needles ethanolic extract of Pinus merkusii on HeLa cell lines
1
Doctoral Program in Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, 60115 Surabaya, Indonesia
2
Department of Pharmacology, Faculty of Veterinary Medicine, Universitas Airlangga, 60115 Surabaya, Indonesia
3
Department of Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, 60115 Surabaya, Indonesia
4
Research Center for Vaccine Technology and Development, Institute of Tropical Disease, Universitas Airlangga, 60115 Surabaya, Indonesia
5
Department of Health, Faculty of Vocational Studies, Universitas Airlangga, 6011 5 Surabaya, Indonesia.
* Corresponding author: annise.drh@gmail.com
Several efforts have been made to discover new anticancer agents based on natural ingredients. Meanwhile, previous studies have shown that different Pine genus species exhibit cytotoxic activity against various types of cancer cells. This plant is rich in phenolic compounds, especially procyanidins, flavonoids, and phenolic acids. Therefore, this study aims to investigate the in vitro cytotoxicity of Pinus merkusii needles extract on HeLa cancer cell lines. The cytotoxicity assessment was measured using MTT assay and expressed as IC50 value. The results showed that the ethanolic extract poses a dose and time-dependent cytotoxic activity with an IC50 value of 542.5 µg/ml at 48 hours of incubation. Based on this result, Pinus merkusii needles’ ethanolic extract has the potential of a novel candidate for an anticancer agent.
© The Authors, published by EDP Sciences, 2021
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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