Re-epithelization and density of collagen fibers on wound healing of mice’s skin (Mus musculus) that treated with combination of chitosan membrane and eel (Monopterus albus) mucous

¹ Undergraduate student, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna, No. 2, Karangmalang, Catur Tunggal, Depok, Sleman, Daerah Istimewa Yogyakarta, Indonesia. 55281
² Undergraduate student, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Jl. Farmako Sekip Utara, Sleman, Daerah Istimewa Yogyakarta, Indonesia. 55281
³ Anatomy Department, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna, No. 2, Karangmalang, Catur Tunggal, Depok, Sleman, Daerah Istimewa Yogyakarta, Indonesia. 55281

^* Corresponding author: estifkh@ugm.ac.id

Abstract

Untreated skin wound can inhibit the wound healing process. Chitosan and eel mucous have biodegradable, biocompatible, and antimicrobial properties. This study aimed to determined the potential of combination between chitosan membrane and eel mucous extract in skin wound re-epithelization and collagen fibers deposition. Thirty adult male mice were divided into 5 groups, 6 animals/group. Skin wo und were perform by punch biopsy in 0.5 cm diameter. Group I-IV respectively recieved: 10% povidone iodine; eel mucus extract and gauze; combination of 1 % chitosan membrane and eel mucus; 1% chitosan membrane. Group V without therapy. Therapy was given once a day for 10 days. At the 5^th and 10^th half of animals/group were euthanized. Wound skin area were collected for hematoxylin-eosin and Mallory-azan stainings. Epithelial thickness and collagen density were observed and analyzed statistically. The result s showed significant difference in epithelial thickness on the 5th and 10th days (P ≤ 0.05) in combination group. Percentage of collagen density at 10th day of combination group showed significantly difference (P ≤ 0.05) compared with control. The conclusion is combination of 1 % chitosan membrane and eel mucous extract has the most potential to re-epithelization and increase density of collagen fibers in skin wound healing.