The effectiveness of the use of the silicon-containing preparation Siliplant in the case of clonal micropropagation of the rose Reine Sammut

¹ FSBSI Udmurt Federal Research Center of the Ural Branch of the Russian Academy of Sciences, Izhevsk, Russia
² FSBEI HE Izhevsk State Agricultural Academy, Izhevsk, Russia

^* Corresponding author: t.lekontseva@yandex.ru

Abstract

Clonal micropropagation of plants is a modern popular method of vegetative reproduction, which allows to obtain high-quality planting material in a large volume in a short time. The research is devoted to the technology of clonal micropropagation of the rose Reine Sammut, in particular, optimization of the nutrient medium composition. To increase the efficiency of micropropagation, the silicon-containing preparation Siliplant was added to the composition of the nutrient medium according to the Murashige-Skoog (MS) recipe as an alternative to the complex of trace elements. At the stage of the actual micropropagation with the combined use of 6-benzylaminopurine (6-BAP) at doses of 1.0; 2.0 and 3.0 mg/l and Siliplant at doses of 1.0; 2.0 and 3.0 ml/l, no large differences were found. Nevertheless, the inclusion of Siliplant (1.0 ml/l) and 6-BAP (3.0 mg/l) in the medium contributed to an increase in the reproduction factor by 3.1 pcs./cuttings at LSD₀₅ = 1.0 pcs. In addition, Siliplant at a dose of 1.0 ml/l as part of a hormone-free nutrient medium MS contributed to a significant increase in shoot growth (by 4.2 mm at LSD₀₅ = 3.6), and at a dose of 3.0 ml/l stimulated the development of standard micro-plants of roses.