Obtaining “ghosts” of red blood cells with a set ion concentration as models for studying Na+, K+ - ATPase

¹ Kursk Federal Agrarian Scientific Center, 70b Karl Marx Street, Kursk, 305021, Russian Federation
² Kursk State Agricultural Academy of I. I. Ivanov, 70, Karl Marx Street, Kursk, 305021, Russian Federation
³ South-Western State University, 94, 50 Let Oktyabrya str., Kursk, 305040, Russian Federation

^* Corresponding author: ugnoe_nebo@list.ru

Abstract

The article considers the production of red blood cell “ghosts” and their application as a model for studying Na+, K+ - ATPase. Various variants of liposomes are used as test objects for studying ion transport, but the best model of biological membranes for studying ion transport and ATPase pumps are the «ghosts» of red blood cells. The activity of blood Na+/K+ - ATPase is determined mainly in the suspension of red blood cell “ghosts”. This is due to the need to remove the interfering compound – hemoglobin - and obtain “ghosts” that are necessary for accurate measurement of the activity of the enzyme. In this scientific work, studies were conducted to determine the concentrations of Na+ and K+ ions in the blood and red blood cells of cows and calves of various ages by atomic absorption and atomic emission spectroscopy. The values of these concentrations of Na+ and K+ ions were used to obtain red blood cell “ghosts”. “Ghosts” of red blood cells with a set concentration of sodium and potassium ions were obtained by the method of J. T. Dodge, in the modification of Zhumadilov Zh. Sh. and Gening T. P. and in our modification. Studies of the concentration of Na+ and K+ ions in the obtained «ghosts» of red blood cells showed their full compliance with the calculated values.