An innovative modification of the nutrient medium formulation for the isolation and differentiation of enterobacteria

Samara State Agrarian University, 2, Uchebnaya str., Ust-Kinelsky, Kinel, 446442, Russia

^* Corresponding author: Vladimir_21_2010@mail.ru

Abstract

In laboratory conditions, the modified nutrient differential diagnostic medium Drigalski agar with lactose is recommended to be used for cultivation (isolation) and differentiation of enterobacteria of the Enterobacteriaceae family. The differentiation of enterobacteria on a modified medium is carried out according to their ability to ferment lactose, mannitol, glucose, sucrose, gelatin and form hydrogen sulfide. The environment can also be used for conducting sanitary and microbiological studies of environmental objects. The environment can be used to perform the ONPG test. In the course of preparing the modified Drigalski agar for operation in laboratory conditions, we recommend using the medium according to one of the options: when one of the carbohydrates is added, the medium is poured into single-section reusable or disposable petri dishes; when two carbohydrates are added, the medium is poured into two-section reusable or disposable petri dishes; when a complex of carbohydrates lactose + mannitol and glucose + sucrose is added, the medium is poured into two-section reusable or disposable petri dishes; when using all four drives separately, the medium is dispensed into single-compartment reusable or disposable petri dishes. We recommend preparing modified Drigalski agar at a plant for the production of ready-made culture media with filling the medium in two-section petri dishes (complex of carbohydrates lactose + mannitol in one section, glucose + sucrose in another section), or with filling the medium in four-section petri dishes (with each carbohydrate in separate section).