| Issue |
BIO Web Conf.
Volume 40, 2021
III International Symposium “Innovations in Life Sciences” (ILS 2021)
|
|
|---|---|---|
| Article Number | 01005 | |
| Number of page(s) | 5 | |
| Section | Fundamental and Applied Aspects of Biology and Biotechnology | |
| DOI | https://doi.org/10.1051/bioconf/20214001005 | |
| Published online | 20 December 2021 | |
Validation of the method for determining the specific activity of the gel with recombinant endolysin
1
G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology, 125212 Moscow, Russia
2
Sechenov First State Medical University, 119435 Moscow, Russian Federation
3
The Gamaleya National Center of Epidemiology and Microbiology, 123098 Moscow, Russian Federation
* Corresponding author: vorobjew.alex2010@yandex.ru
A method for determining the specific bactericidal activity of the gel with the recombinant modified endolysin of the bacteriophage ECD7 has been developed. Bacterial strains to determine the specific bactericidal activity were selected and acceptance criteria were determined. The developed methodology was validated according to the indicators “Specificity”, “Accuracy” and “Intermediate precision”. The developed method has been successfully validated and is suitable for determining the specific bactericidal activity of a gel with a recombinant modified endolysin.
Key words: bactericidal activity / recombinant endolysin / bacteriophage ECD7
© The Authors, published by EDP Sciences, 2021
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