BIO Web Conf.
Volume 40, 2021III International Symposium “Innovations in Life Sciences” (ILS 2021)
|Number of page(s)||6|
|Section||Fundamental and Applied Aspects of Biology and Biotechnology|
|Published online||20 December 2021|
Intraspecies variability of the 16S rRNA gene of the soil bacteria Acinetobacter lwoffii and Paenibacillus taichungensis
Belgorod State National Research University, 308015, Belgorod, Russia
* Corresponding author: email@example.com
The functioning of well-studied key groups of soil microorganisms depends on the microbial ecosystem in which they function in interaction with species belonging to other ecological groups. An analysis of the composition of the soil microbiota, and the possibility of analyzing the number of representatives of specific species, remains an urgent task of soil microbiology. Such analysis could be performed using modern and expensive methods such as metagenomics and mass-spectrometry, but some questions could also be answered using real-time PCR. This well-known approach is rather cheap for massive analysis and is ready to present reproducible results for practical agricultural applications. Understanding the variability of the primary structure of 16S rRNA is key to the reliable identification of bacterial species and provides an opportunity to choose the optimal pathways for their detection by PCR. In this work, analysis of the sequences of 16S rRNA of two species of soil bacteria, Acinetobacter lwoffii and Paenibacillus taichungensis is carried out. The most variable and most conservative areas on the level of species are detected. It was proved that conventional variable and conservative areas of the gene have on average nearly the same level of variability on the intraspecies level.
Key words: Acinetobacter lwoffii / Paenibacillus taichungensis / variability of 16S rRNA
© The Authors, published by EDP Sciences, 2021
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