| Issue |
BIO Web Conf.
Volume 42, 2022
International Scientific and Practical Conference “Sustainable Development of Traditional and Organic Agriculture in the Concept of Green Economy” (SDGE 2021)
|
|
|---|---|---|
| Article Number | 01014 | |
| Number of page(s) | 7 | |
| Section | Agrobiotechnology in Crop and Livestock Production | |
| DOI | https://doi.org/10.1051/bioconf/20224201014 | |
| Published online | 13 January 2022 | |
Strategies for Reducing Ruminant Methane Emissions
Federal Research Centre of Biological Systems and Agrotechnologies, RAS, Orenburg, Russia
The paper studies the effect of additional administration of ultrafine particles on the cattle rumen microbiome composition. The in vitro method was used using the ANKOM Daisy II incubator according to a specialized method. Microflora analysis was performed using MiSeq (Illumina, USA) by a new generation sequencing method with a MiSeq reagent kit. After a detailed analysis of the structure and composition of the microbial community in the contents of the rumen sampled for different diets, it was found that no significant differences were observed in the bacterial communities, with the exception of a slight shift in the Bacteroidetes/Firmicutes ratio. However, we observed numerical differences in the abundance of some representatives, namely, with additional inclusion of Fe and Cr2O3, decrease in the abundance of the methane-forming species Methanobrevibacter, Methanobacterium, Methanosphaera, and Methnaomicrobium was noted regarding the control.
© The Authors, published by EDP Sciences, 2022
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