BIO Web Conf.
Volume 42, 2022International Scientific and Practical Conference “Sustainable Development of Traditional and Organic Agriculture in the Concept of Green Economy” (SDGE 2021)
|Number of page(s)||6|
|Section||Agrobiotechnology in Crop and Livestock Production|
|Published online||13 January 2022|
Genetic variation of CAST gene in Local Karnobat and Karnobat merino sheep breeds
Agronomy Faculty, University of Forestry, Sofia, Bulgaria
2 Institute of Animal Science, Kostinbrod, Bulgaria
3 Institute of Agriculture, Karnobat, Bulgaria
* Corresponding author: email@example.com
Karnobat sheep plays an important role in the development of sheep breeding in Southeastern region of Bulgaria. They are valuable source of genetic material. The aim of present experiment was to determine the allele variation of CAST gene in Local Karnobat and Karnobat Merino sheep breeds. A total of 60 blood samples were collected – 30 per breed. DNA was extracted and genotypes of all animals were identified by means of PCR-RFLP technique. The restriction reactions were accomplished by specific enzyme MspI. As expected both breeds were characterized with low level of genetic diversity due to the fact that mostly maintaining selection has been implemented. In Local Karnobat sheep breed was identified only one heterozygous individual from all 30. In Karnobat merino were identified allele M with frequency 0,97 and allele N with frequency 0,03. Genotypes MM and MN were revealed with frequencies 0,93 and 0,07, respectively. According to the statistical analysis both breeds were in HWE equilibrium.
© The Authors, published by EDP Sciences, 2022
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