| Issue |
BIO Web Conf.
Volume 43, 2022
International Scientific and Practical Conference “VAVILOV READINGS-2021” (VVRD 2021) dedicated to the 101st anniversary of the discovery of the law of homological series and the 134th anniversary of the birth of N. I. Vavilov
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|---|---|---|
| Article Number | 01002 | |
| Number of page(s) | 12 | |
| Section | Basic Research in the Field of Plant and Microbial Studies | |
| DOI | https://doi.org/10.1051/bioconf/20224301002 | |
| Published online | 19 January 2022 | |
Molecular and morphological traits of Pyricularia oryzae Cav. in the south of Russia
1 Federal Scientific Rice Centre, 350921, Belozerny, 3, Krasnodar, Russian Federation
2 Kuban State Agrarian University named after I.T. Trubilin, 350004, Kalinina, 13, Krasnodar, Russian Federation
3 All-Russian Research Institute of Agricultural Biotechnology, 127550, 42, st. Timiryazevskaya, Moscow, Russian Federation
* Corresponding author: lenakrug1@rambler.ru
Based on molecular genetic approaches, a study of the biodiversity of the phytopathogenic fungus Pyricularia oryzae Cav. was carried out in the south of Russia, the classification of morphological and cultural traits of the pathogen was carried out. In 2020, monitoring was carried out and 9 strains of the pathogen were isolated from the affected herbarium material collected from the fields of five ecological rice-growing zones of Krasnodar region (Krasnoarmeisky, Kalininsky, Abinsky, Slavyansky districts, Krasnodar). Using a multiplex PCR system based on fragment analysis, among the studied strains of Pyricularia oryzae Cav. 5 genotypes were identified in 2020, each of which is was characterized by a unique genetic profile. Their «DNA-passports» have been compiled. The studied isolates of the blast pathogen were characterized by morphological and cultural traits. 6 morphotypes of the pathogen were identified. Classification of the pathogen by morphological, cultural and genetic traits will allow obtaining scientifically grounded and comparable data that can be used in the analysis of the interaction of blast strains with rice plants on different varieties and in different agro-technological conditions in order to improve and rationalize agricultural activities. In addition, the studied approaches open up the possibility of using data in breeding, allowing the identification of pathogen forms that infect certain varieties.
© The Authors, published by EDP Sciences, 2022
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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