| Issue |
BIO Web Conf.
Volume 43, 2022
International Scientific and Practical Conference “VAVILOV READINGS-2021” (VVRD 2021) dedicated to the 101st anniversary of the discovery of the law of homological series and the 134th anniversary of the birth of N. I. Vavilov
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| Article Number | 03007 | |
| Number of page(s) | 8 | |
| Section | Biotechnologies | |
| DOI | https://doi.org/10.1051/bioconf/20224303007 | |
| Published online | 19 January 2022 | |
PCR-RFLP analysis of rbcL chloroplast gene
Volga State University of Technology, Forestry Department, 424000, 3, Lenin Square, Yoshkar-Ola, Republic of Mari El, Russian Federation
* Corresponding author: ShejkinaOV@volgatech.net
To identify intergeneric, interspecific, and intraspecific polymorphisms, PCR-RFLP studies of the chloroplast rbcL gene profiles were carried out using five restriction endonucleases (AluI, HpaII, BsuRI, BstHHI, BstMBI) in 14 species belonging to 6 different genera and 7 Thuja occidentalis cultivars. For all the genera studied (Pinus sp., Abies sp., Picea sp., Microbiota sp., Syringa sp., Thuja sp.), A genus-specific restriction profile characterized by a unique combination of restriction DNA fragments was revealed. The combination of enzymes used in the study did not reveal the interspecies and intraspecific variability of the rbcL gene. Species belonging to the same genus and all varieties of western thuja were characterized by identical PCR-RFLP profiles. It is assumed that further research, with the inclusion of a larger number of species and enzymes, will reveal polymorphism at the interspecies and intraspecific levels.
© The Authors, published by EDP Sciences, 2022
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