| Issue |
BIO Web Conf.
Volume 43, 2022
International Scientific and Practical Conference “VAVILOV READINGS-2021” (VVRD 2021) dedicated to the 101st anniversary of the discovery of the law of homological series and the 134th anniversary of the birth of N. I. Vavilov
|
|
|---|---|---|
| Article Number | 03042 | |
| Number of page(s) | 6 | |
| Section | Biotechnologies | |
| DOI | https://doi.org/10.1051/bioconf/20224303042 | |
| Published online | 19 January 2022 | |
Creation of Antibody Immunoassay Test Systems for Indication of Enteropathogenic Yersinia
Saratov State Agrarian University named after N.I. Vavilova, 1, Teatralnaya Square, Saratov, 410600, Russian Federation
* Corresponding author: ivashenko-sv@mail.ru
3 immunoassay test systems based on antibodies obtained as a result of immunization of rabbits with one of the antigens: disintegrated membranes (DM) Y. enterocolitica, DM Y. pseudotuberculosis and lipopolysaccharide Y. pseudotuberculosis were created. The best diagnostic properties were possessed by a test system based on antibodies to Y. pseudotuberculosis DM. It showed high titers of antibodies to various serovars of Y. pseudotuberculosis (1:25600-1:51200), incl. to microbe strains isolated from farm animals, and low titers to Y. enterocolitica (1:200). In the accumulation media with pig excrements seeded with Yersinia, the experimental test system detected Y. pseudotuberculosis already on the 3rd day of “cold enrichment” provided that 50 or more Yersinia cells per ml were inoculated into the accumulation medium together with excrements.
© The Authors, published by EDP Sciences, 2022
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.