| Issue |
BIO Web Conf.
Volume 46, 2022
International Scientific Online-Conference “Directed Transformation of Alimentary Raw Materials in the Production of Foodstuffs, Food and Biologically Active Additives, Ensuring Quality Control and Safety” (DTARM 2022)
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|---|---|---|
| Article Number | 01008 | |
| Number of page(s) | 5 | |
| DOI | https://doi.org/10.1051/bioconf/20224601008 | |
| Published online | 22 April 2022 | |
Research of the survival of a consortium of Zygosaccharomyces kombuchaensis yeast and Gluconoacetobacter xylinus bacteria during frozen storage using various protective media
Krasnodar Research Institute of Agricultural Products Storage and Processing – branch of FSBSO «North-Caucasian Federal Scientific Center of Horticulture & Viniculture», st. Topolinaya alleya, 2, Krasnodar, 350072, Russia
* Corresponding author: wuhdz@mail.ru
In the present study the survival of the consortium of yeast and bacteria using protective media was investigated. The protective medium consisting of 1 % gelatin, 5 % monosodium glutamate and 5 % sucrose provides the best preservation of living cells of the consortium and allows stabilization of cells for up to 100 days at storage temperatures from -2 °C to -10 °C. The projective medium consisting of 1 % gelatin and 10 % sucrose provides effective storage of the cells at t=-2 °C for 100 days; t=-5 °C – 80 days; t=-10 °C – 60 days. The projective medium of skimmed milk and 7.5 % glucose ensures effective storage of the cells of the consortium at t=-2 °C for 100 days; t=-5 °C – 80 days; t=-10 °C – 80 days. The information obtained is interesting for following researches of the development of technologies for the bioconversion of plant materials.
© The Authors, published by EDP Sciences, 2022
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