BIO Web Conf.
Volume 51, 2022International Scientific and Practical Conference “From Modernization to Advanced Development: Ensuring Competitiveness and Scientific Leadership of the Agro-Industrial Complex” (IDSISA 2022)
|Number of page(s)||8|
|Section||Innovative Technologies for the Production and Processing of Crop and Livestock Products: Problems and Prospects for Practical Implementation|
|Published online||02 August 2022|
Molecular research in the tomato breeding for resistance to biotic stressors
1 FSBSI «Federal Scientific Rice Centre», 350921, Krasnodar, Belozerny, 3, Russia
2 FSBEI HE «Kuban State Agrarian University named after I.T. Trubilin», 350044, Krasnodar, Kalinina, 13, Russia
* Corresponding author: email@example.com
An urgent problem in tomato breeding is the development of universal varieties and hybrids that combine high yields and good fruit quality with group resistance to a number of diseases. The purpose of this study is to develop tomato breeding material resistant to biotic stressors, basing on modern postgenomic technologies using molecular markers. At the initial stage, we tested microsatellite markers from foreign literature sources, the primers used in the study were synthesized by Syntol LLC, samples from the collection of FSBSI Federal Scientific Center for Biological Plant Protection and FSBSI Federal Scientific Rice Centre were studied under the conditions of Krasnodar region. PCR conditions for identifying the target Ph -3 gene in the tomato plant material used in the breeding process were optimized. Thus, in our study on the testing of microsatellite markers to identify the target Ph-3 resistance gene in the tomato genetic material, we selected 3 informative microsatellite markers R2M1S, G8-1, R1-3U, which reveal the allelic difference between susceptible and resistant samples.
© The Authors, published by EDP Sciences, 2022
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