| Issue |
BIO Web Conf.
Volume 53, 2022
International Scientific-Practical Conference “Modern Trends of Science, Innovative Technologies in Viticulture and Winemaking” (MTSITVW2022)
|
|
|---|---|---|
| Article Number | 02001 | |
| Number of page(s) | 8 | |
| Section | Genetic Resources, Genetics, Genomics, Bioengineering | |
| DOI | https://doi.org/10.1051/bioconf/20225302001 | |
| Published online | 10 November 2022 | |
Chloroplast genomes of Vitis sylvestris Gmel. samples from Damanskaya population of the Krasnodar region
1 Laboratory of Plant and Animal genetics, Kuban State Agrarian University named after I.T. Trubilin, 350044 Kalinina 12, Krasnodar, Russian Federation
2 Academy of biology and biotechnology, Southern Federal University, 344090 Rostov-on-Don, Stachki Av., 194/1, Russian Federation
3 Department of Viticulture, Kuban State Agrarian University named after I.T. Trubilin, 350044 Kalinina 12, Krasnodar, Russian Federation
* Corresponding author: milovanov1991@mail.ru
This article presents the structures of chloroplast genomes of three Vitis sylvestris Gmel. samples from Damanskaya population of the Krasnodar Territory. An expedition to the place where wild forest grapevine grows was made. After that, the selected leaves served as a source of chloroplasts. DNA was isolated from them and DNA libraries were prepared and sequenced. Genome assembly was carried out after selection of the most suitable reference sample at coverage of 35.0x. The genomes ranged in size from 159,900 to 160,887. Aligned chloroplast genomes were annotated with GeSeq and GeneMark.hmm. OGDRAW was used to visualize the structure of the genomes. GenBank search allowed to determine their belonging to V. sylvestris species. At the same time, a comparison of the genomes with each other showed the presence of minor differences in their structure.
© The Authors, published by EDP Sciences, 2022
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0 (http://creativecommons.org/licenses/by/4.0/).
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