Features of somatic embryogenesis in the culture in vitro in hybrid grape form E-342

All-Russian National Research Institute of Viticulture and Winemaking Magarach of the RAS, 31 Kirova Str., 298600 Yalta, Republic of Crimea, Russia

Abstract

Correlation analysis of the influence of media variants with introducing various biologically active substances on the development of somatic embryoids and germinating seedlings in the hybrid form E-342 was carried out during two repeated subculturings on the same variant of media, and during the third subculturing - on different variants of media. The repeated subculturing in media with the same growth regulator BAP or IAA caused the development of abnormal large somatic embryoids without the expressed hypocotyls and cotyledons. Only the use of medium variant with DLPA in two subculturings (the third subculturing into the medium with BAP or IAA) and the third repeated subculturing with DLPA, but to a smaller extent, have led to the development of torpedo-shaped embryoids, to be growing into germinating seedlings. Strong positive correlation dependence of influence was established for: BAP on the development of heart-shaped embryoids; IAA on the increase in the size of globular embryoids and transformation of heart-shaped embryoids into torpedo-shaped ones; DLPA on the development of a great number of normal torpedo-shaped embryoids. After longstanding culturing of suspensions of cells and embryoids, it was not possible to get rid of chlorophyll deficiency in the resulting germinating seedlings due to somaclonal variability.