BIO Web Conf.
Volume 55, 20225th International Conference on Frontiers of Biological Sciences and Engineering (FBSE 2022)
|Number of page(s)
|21 November 2022
Determination of Taurine in Quadriceps Femoris by High Performance Liquid Chromatography with Precolumn Derivatization
1 College of seed and facility agricultural engineering, Weifang University, Weifang, Shandong, China
2 Shandong Vocational Animal Science and Veterinary College, Weifang, Shandong, China
3 College of Biology and Oceanography, Weifang University, Weifang, Shandong, China
* Corresponding author: firstname.lastname@example.org
A method for determination of taurine in mouse quadriceps femoris by high performance liquid chromatography with precolumn derivatization ophthalaldehyde (OPA) was established. The sample was extracted and treated with canon exchange resin. Results showed that taurine in quadriceps femoris was separated and quantified on C18 reversed phase column by high performance liquid chromatographic (HPLC) after derivatization with OPA in 3min, using mixture of methanol and phosphate （V/V＝1:1，pH＝4.9） as mobile phase, rate of flow is 0.6mL/min, detecting at 340nm by UV-detector, L-Glutamine as internal standard. The result showed that the linear ranger of taurine was 6.25-187.7ng/mL, correlation coefficient R2=0.9994, the recoveries were 91.8%-101.8%, RSD=3.2% (n=6). The retention time of taurine is 7.32 min. The concentration of taurine in mouse quadriceps femoris is 3.18mg/g. The protein and amino acid were separated by sample pre-treatment. The method is of good separation effect, simple, reliable, and can be used to analyze the taurine concentration in mammal tissue.
© The Authors, published by EDP Sciences, 2022
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