| Issue |
BIO Web Conf.
Volume 57, 2023
International Scientific and Practical Conference “Innovations, Technological Solutions and Management in Modern Biotechnology and Biomedicine” (ITSM-2022)
|
|
|---|---|---|
| Article Number | 03003 | |
| Number of page(s) | 6 | |
| Section | Pharmaceutical Biotechnology | |
| DOI | https://doi.org/10.1051/bioconf/20235703003 | |
| Published online | 13 January 2023 | |
Development of Clonal Micropropagation Technology for Ludisia Discolor (Ker Gawl.) A. Rich. In Vitro Conditions
1 V.I. Vernadsky Crimean Federal University, Simferopol, Russia
2 Komarov Botanical Institute of the Russian Academy of Sciences, St. Petersburg, Russia
* Corresponding author: vvn22222@mail.ru
The features of clonal micropropagation in three variations of Ludisia discolor were studied. Brood buds, apical shoot meristems, immature seeds and mature seeds were used as explants. They are grown using Murashige & Skoog modified medium. A special technique has been developed for the sterilization and planting of microscopic mature seeds. Mature seeds germinated for 60-70 days. Seeds from 25-day-old capsules showed the best results among immature seeds. They gave mass shoots already on the 25-30 day after planting. The roller of the future first leaf of the protocorms was laid in the dark 2 months after germination. Switching to the light mode induced rapid gemmorizogenesis - the formation of the first green leaves, stem and adventitious roots. Seeds from 15-day-old capsules did not germinate at all. Planting with immature seeds is the most effective method of clonal reproduction of L. discolor in vitro.
© The Authors, published by EDP Sciences, 2023
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