BIO Web Conf.
Volume 59, 20232023 5th International Conference on Biotechnology and Biomedicine (ICBB 2023)
|Number of page(s)||5|
|Section||Biotechnology and Cell Structure Analysis|
|Published online||08 May 2023|
Evaluation of Biosynthetic Pathway and Engineered Biosynthesis of Morphine with CRISPR
College of Arts and Sciences, The Ohio State University, Columbus, Ohio, 43212, United States
As a secondary metabolite produced by opium poppy (Papaver somniferum), morphine is a valuable subject for research and application purposes within the medical field. Thanks to the research conducted throughout the past few decades, the main pathway for the biosynthesis of morphine has been thoroughly elucidated. In addition, whole genome sequencing and evolutionary genomics of opium poppy have provided us with crucial information regarding specific details of such a pathway on the molecular level, which includes coding regions and functions of key enzymes that play vital roles in the production of specific metabolites. These advantages can be combined with the application of current genome editing techniques, such as CRISPR, to allow the regulated and optimized production of desirable metabolites through manipulating particular genes. This research focuses on the functional evaluation of key enzymes in the biosynthetic pathway of morphine in opium poppy, further exploring possibilities of regulated production of morphine with CRISPR.
© The Authors, published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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