| Issue |
BIO Web Conf.
Volume 63, 2023
II International Conference “Problems of Nature Management and Environmental Protection” (ASE-2023)
|
|
|---|---|---|
| Article Number | 07003 | |
| Number of page(s) | 5 | |
| Section | Natural Resource Potential and Ecological Rehabilitation of Degraded Landscapes | |
| DOI | https://doi.org/10.1051/bioconf/20236307003 | |
| Published online | 21 July 2023 | |
The Use of RAPD Markers in the Study of Polymorphism of Mountain Populations of Dandelion Officinalis
1 Kadyrov Chechen State University, Sheripova Street, 32, 364024, Grozny, Russia
2 Kh. Ibragimov Complex Institute of the Russian Academy of Sciences (CI RAS), Leninsky Ave., 14, 119991, Moscow, Russia
* Corresponding author: petimat-lg@rambler.ru
Molecular markers are used in plant science in a variety of ways. The method of molecular genome marking based on RAPD-PCR makes it possible to determine the genetic status of populations and establish intrapopulation relationships. In this work, we studied the genetic polymorphism of the dandelion Taraxacum officionale using RAPD markers. Five markers of random amplified polymorphic DNA (RAPD) were used to determine the genetic diversity and genetic relationship between two populations of T. officionale growing on the slopes of the Andean Range. A total of 26 fragments were amplified, and 25 of them were polymorphic, with an average of 6 amplified bands per primer. The percentage of total polymorphism was 96.1%. The size of the amplified fragments varied from 150 to 1200 bp. It has been shown that molecular genetic analysis makes it possible to identify RAPD markers of the dandelion genome, which can be used for population identification of genotypes.
© The Authors, published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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